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《Carcinogenesis,Teratogenesis and Mutagenesis》 1993-01
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Cai Ping, Wang Jinying, Chen Tiehe, Xiang Zhenghua, Mong Lin, Wang Xinmin, Li Huaiyi Naval Medical Research Institute, Shanghai 200433 Department of Histology and Embryology, Department of Biology Second Military Medical University  
The DNA fragments microdissected from 1 region of the salivary gland X chromosome of D.melanogaster were cloned into PGEM3Z plasmid. From more than 10pg of chromosome DNA, 9 recombinent clones were obtained. The five recombinent clones were harvested and analysed.The two recombinent fragments are about 0.6 kb, the other three recombinent fragments are about 3.0 kb. The two RNA probes were synthesed with DNA dependent SP6 RNA polymerase translation system in vitro from the two recombinet clones containing fragements about 0.6 kb, and locateded at the region 1E and 1F of the X chromosome of D.melanogaster by hybridization in situ.The results showed that with this method, RNA can be synthesized in vitro directory from the recombinant clones which contain the DNA fragments from the D.melarogaster chromosome.
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1 LIU Yu-ling et al(Cotton Research Institute,Chinese Academy of Agricultural Sciences / Key Laboratory of Cotton Genetic Improvement of Ministry of Agriculture,Anyang,Henan 455000);Research Advances of Chromosome Micro-dissection Technology and Its Utilization in Plants[J];Journal of Anhui Agricultural Sciences;2010-16
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8 LU Liu xin 1, HUANG Dai qing 1,2 (1.College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China; 2.College of Bio engineering, Fujian Teachers' University, Fuzhou, Fujian 350002, China);Advances and prospects in microdissection and microcloning of plant chromosomes[J];Journal of Fujian Agricultural University(Natural Science);2001-03
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1 GENG Ke-xiang ,YAN Shou-qing, LI Bing,SUN Jin-hai (College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China; College of Animal Science & Technology, Laiyang Agricultural University, Qingdao 266109, China);Microdissection and Microcloning of Swine 13/17Robertsonian Translocation Chromosome[A];[C];2006
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