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《Carcinogenesis,Teratogenesis and Mutagenesis》 1993-01
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THE PREPARATION OF RNA PROBES WITH CLONING OF DNA FROM MICRODISSECTED D.MELANOGASTER CHROMOSOME FRAGMENTS AND ITS LOCALIZATION BY HYBRIDIZATION IN SITU

Cai Ping, Wang Jinying, Chen Tiehe, Xiang Zhenghua, Mong Lin, Wang Xinmin, Li Huaiyi Naval Medical Research Institute, Shanghai 200433 Department of Histology and Embryology, Department of Biology Second Military Medical University  
The DNA fragments microdissected from 1 region of the salivary gland X chromosome of D.melanogaster were cloned into PGEM3Z plasmid. From more than 10pg of chromosome DNA, 9 recombinent clones were obtained. The five recombinent clones were harvested and analysed.The two recombinent fragments are about 0.6 kb, the other three recombinent fragments are about 3.0 kb. The two RNA probes were synthesed with DNA dependent SP6 RNA polymerase translation system in vitro from the two recombinet clones containing fragements about 0.6 kb, and locateded at the region 1E and 1F of the X chromosome of D.melanogaster by hybridization in situ.The results showed that with this method, RNA can be synthesized in vitro directory from the recombinant clones which contain the DNA fragments from the D.melarogaster chromosome.
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【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LIU Yu-ling et al(Cotton Research Institute,Chinese Academy of Agricultural Sciences / Key Laboratory of Cotton Genetic Improvement of Ministry of Agriculture,Anyang,Henan 455000);Research Advances of Chromosome Micro-dissection Technology and Its Utilization in Plants[J];Journal of Anhui Agricultural Sciences;2010-16
2 JI Fu-Yun YU Qi-Xing **- GUO Yi-Qing HAI Yan\ ZHOU Rong-Jia\ LIU Li (College of Life Science, Wuhan University, Wuhan 430072, China);MICROISOLATION AND CHARACTERIZATION OF SINGLE CHROMOSOME IN RICE FIELD EELS (MONOPTERUS ALBUS)[J];Acta Zoologica Sinica;2002-01
3 YANG Hong-yan, WANG Jun, KANG Xiang-yang National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, Beijing Forestry University, Beijing 100083, P. R. China;Meiotic chromosome preparation techniques of pollen mother cells for laser micro-dissection in Populus spp.[J];中国林学(英文版);2010-02
4 Cui Lihua 1) Chen Zhenghua 2) ( 1)College of Life Sciences, Beijing Normal University, 100875, Beijing, China; 2)Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, China);AMPLIFICATION OF MICRODISSECTED CHROMOSOMES FROM VICIA FABA BY TWO PCR TECHNIQUES[J];Journal of Beijing Normal University(Natural Science);2004-02
5 WANG Li-ming1,2,ZHU Yu-li2,LI Xing-feng2,LIU Shu-bing2,WANG Hong-gang2(1 Agronomy College of Henan University of Science and Technology,Luoyang,He'nan 471003,China;2 Agronomy College of Shandong Agricultural University,Shandong Subcentre of National Wheat Improvement Centre,State Key Laboratory of Crop Biology,Tai'an,Shandong 271018,China);Research Advances of Chromosome Microdissection and Microcloning Technology and Its Utilizations in Plant[J];Acta Botanica Boreali-Occidentalia Sinica;2007-05
6 Chai Jianhua, (Institute of Genetics);MOLECULAR CLONING AND RESTRICTION MAPPING OF TANDEMLY REPETITIVE SEQUENCE OF Acetabularia mediterranea CHLOROPLAST GENOME[J];Journal of Fudan University;1990-04
7 Deng Kejing, He Fenghua, Shen Daleng (Institute of Genetics);Microdissection and Amplification of Rice Chromosome No.4[J];JOURNAL OF FUDAN UNIVERSITY;1999-04
8 LU Liu xin 1, HUANG Dai qing 1,2 (1.College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China; 2.College of Bio engineering, Fujian Teachers' University, Fuzhou, Fujian 350002, China);Advances and prospects in microdissection and microcloning of plant chromosomes[J];Journal of Fujian Agricultural University(Natural Science);2001-03
9 WU Jing-hua~1, HUANG Dai-qing~2, LU Liu-xin~3, ZHANG Zhi-zhong~3(1.College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China; 2.Bioengineering College, Fujian Teachers University, Fuzhou, Fujian 350007, China; 3.College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China);Microdissection of the single chromosome in Narcissus[J];;2003-04
10 GENG Ke-xiang1,YAN Shou-qing1,LI Bing1,SUN Jin-hai1,2(1.College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China;2.College of Animal Science and Technology,Laiyang Agricultural University,Qingdao 266109,China);Microdissection and LA-PCR Amplification of Swine Single 13/17 Robertsonian Translocation Chromosome[J];China Animal Husbandry & Veterinary Medicine;2007-04
China Proceedings of conference Full-text Database 1 Hits
1 GENG Ke-xiang ,YAN Shou-qing, LI Bing,SUN Jin-hai (College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China; College of Animal Science & Technology, Laiyang Agricultural University, Qingdao 266109, China);Microdissection and Microcloning of Swine 13/17Robertsonian Translocation Chromosome[A];[C];2006
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