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《CARCINOGENESIS, TERATOGENESIS AND MUTAGENESIS》 2000-01
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STUDY OF EFFECT OF H_2O_2 ON CONTENT OF 8-HYDROXYGUANINES IN HL-60 CELLULAR DNA

ZHANG Hai tao, ZHU Qi feng, MO Li er, ZHUANG Hai qi, Cai Chun (Institute of Medical Biochemistry, Guangdong Medical College, Zhanjiang 524023, China)  
Purpose and Methods: The 8-hydroxyguanine is one of oxidized productions in DNA . In this report, the HL-60 cells were treated with varying concentration H\-2O\-2 and different time. Content of 8-hydroxyguanines in DNA was detected by GC/FID, CGC/MS-SIM,yields of MDA and ratio of GSH/GSSG were measured in HL-60 cells. Results: The results showed that yields of MDA was increased (P0.01), ratio of GSH/GSSG was decreased (P0.01) in HL-60 cells damaged by H\-2O\-2. Detection of GC/FID and GC/MS-SIM indicated the 8-hydroxyguanines be produced in HL-60 cellular DNA. Conclusion: The experiments suggested yields of MDA, ratio of GSH/GSSG and content of 8-hydroxyguanines depend on the concentration of H\-2O\-2 and treatment time.
【Fund】: 广东省重点学科资助课题!960 8
【CateGory Index】: Q27
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