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《Carcinogenesis,Teratogenesis and Mutagenesis》 2001-02
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STUDY ON THE EFFECTS OF DNA CROSSLINKS OF FORMALDEHYDE AND MITOMYCINE C

ZHANG Zun zhen, HENG Zheng chang (Department of Environmental Health, West China University of Medical Sciences, Chengdu 610041, China)  
Purpose: To explore the time course effects in detecting DNA crosslinks with comet assay and to prove the feasibility of comet assay for detecting DNA crosslinks. Method: Hydrogen peroxide (H\-2O\-2) was used as standard DNA breaking agent, the relationship of time effects of DNA crosslinks in TK6 cells induced by formaldehyde (FA) was studied using comet assay. Meanwile, two kinds of DNA crosslinking agents (FA and mitomycine c) were tested and verified. Results: The length of DNA migration caused by H\-2O\-2 decreased with the increase in the dosage of FA and MMC within the doses given. The investigation of time course effects of DNA crosslinks showed that the action of DNA crosslinks increased with increase in the exposure time of FA. Conclusion: The comet assay could detect both DNA protein crosslinks and DNA DNA crosslinks. The comet assay was more sensitive for detecting DNA protein crosslinks than detecting DNA DNA crosslinks.
【Fund】: 国家自然科学基金资助项目!( 3 9670 62 5 )
【CateGory Index】: R99
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