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《Carcinogenesis,Teratogenesis and Mutagenesis》 2001-03
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COMPARISON RESEARCH OF SCGE, SCE AND CHROMOSOME ABERRATION FOR DETERMINING DNA DAMAGE

Ma Ai guo, Zang Jin lin, Song Feng rong, Zhang Ri jin (Institute of Human Nutrition, Medical College of Qingdao University, Qingdao 266021, China)  
Purpose and Methods: Single cell gel electrophoresis(SCGE), sister chromatid exchanges(SCE), chromosome aberrations are three methods to determine DNA damage and repair. The purpose of this study was to compare the sensitivity and accuracy of the three methods. Results: SCGE was the most sensitive one for detecting DNA damage induced by H\-2O\-2. The rates of DNA damage at 100 μmol/L and 200 μmol/L of H\-2O\-2 were 45.6% and 59.5% measured by SCGE, and 3.4% and 5.3% by SCE, respectively. DNA \{damage\} induced by H\-2O\-2 was not detectable with chromosome aberration method. Conclusion: SCGE, SCE and chromosome aberration were considered to be used in three different levels, whereas SCGE was a quick, simple and more sensitive technique for detecting DNA damage and repair.
【Fund】: 山东省教委科研基金! (Z98K5 4)
【CateGory Index】: R99
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