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《Carcinogenesis,Teratogenesis and Mutagenesis》 2004-01
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Simultaneous Detection of the Numerical and Structural Chromosome Aberrations of Sperm in Normal Men by Multi-color Fluorescence in situ Hybridization (FISH)

LIU Xin-xia,HUANG Guo-xian, DENG U-xia?et al ( 1. Center for Disease Control and Prevention of Zhongshan, Zhongshan 528400, China; 2. Lab. of Genetic Toxicology, School of Public Health, Sun Yat - Sen University, Guangzhou 510080, China)  
BACKGROUND AIM: The purpose of this paper was to develop a method of multi - color FISH which could be used to detect the frequencies of numerical and structural chromosomal aberrations in sperm simultaneously. MATERIAL AND METHODS:Two probes for chromosome 1(one for centromere labeled with biotin,the other for terminal of short arm labeled with digoxingenin) and two probes for centromere chromosome 18 labeled with biotin or digoxingenin. The hybridization signals were detected with CY3- strepavidin for botin - labeled probes, and anti -digoxingenin antibodies conjugated with FITC for digoxingenin probes. RESULTS: Under the blue fluorescenses background , three hybridized signals red , green, and yellow , were clearly visible in the sperm nucleus . The red signals represented the target of chromosome 1 ; green signals represented the target of chromosome lp terminal, and yellow signals (which was the mixed color of red and green) represented the centromere of chromosome 18. Three kinds of chromosomal abnormalities were simultaneously detected by the multi - color FISH: the frequencies of disomic and nullisomic sperm for chromosome 1 and 18; the frequencies of duplication and deletion of cetromere , telemere for chromosome 1; and the frequency of diploidy sperms. A total of 135 937 sperm nucleus of 14 normal health men were counted. The frequencies of numerical and structural aberrations were detected. The frequencies of disomic and nullisomic were 0.045 % and 0.048 % for chromosome 1, 0.053 % and 0.045 % for chromosome 18, respectively; the frequencies of duplication and deletion for 1p terminal were 0.082 % and 0.069 % respectively; the frequencies of duplication and deletions for chromosome 1 centromere were 0.075 % and 0.06 0 % respectively; and the frequencies of diploidy sperm was 0.061 %. All the values detected were in the range of the data published in literature . CONCLUSION: Multi - color FISH could be used to detect the numerical and structural chromosome aberrations of sperm in normal men and exposed to environmental chemicals.
【Fund】: 广东省自然科学基金资助项目(No001334)
【CateGory Index】: R446.9
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【References】
Chinese Journal Full-text Database 3 Hits
1 MA Ming-fu,LI Lian-bing,Lü Jing,LI Xin-sheng,CHEN Xiao-xing,ZHAO Le-tian(Institute of Chongqing National Population and Family Planning Science, Chongqing 400020, China);Aneuploidy Detection by Multi-color Fluorescence in Situ Hybridization in Patients with Asthenospermia[J];Carcinogenesis,Teratogenesis & Mutagenesis;2008-03
2 WU Yong-ming ~(1,2),XIA Xin-yi ~(1),HUANG Yu-feng ~(1)1.Laboratory of Reproduction & Genetics,Nanjing General Hospital of Nanjing Command,PLA,Nanjing,Jiangsu 210002,China;2.College of Life Science,Nanjing Normal University,Nanjing,Jiangsu 210097,China;Progress on the Detection Techniques for DNA Integrity of Sperm[J];National Journal of Andrology;2006-08
3 BO Xin,LI Jing-xia (Normal College of Beijing Union University,Beijing 100011,China);Experiments on the common and reproduction toxicities of the Tibet Kefir[J];China Dairy Industry;2007-08
【Citations】
Chinese Journal Full-text Database 3 Hits
1 LI Xin, ZHENG Lü-Kang, DENG Li-Xia ,ZHANG Qiao (Lab of Genetic Toxicology, School of Public Health, Sun Yat-Sen University of Medical Sciences, Guangzhou 510089, China);Detection of Numerical Chromosome Aberrations in Sperm of Workers Exposed to Benzene Series by Two-color Fluorescence in situ Hybridization[J];Acta Genetica Sinica;2001-07
2 ZHENG Lukang *, LIU Shengqin, DENG Lixia, ZHANG Qiao.*Laboratory of Genetic Toxicology, Sun Yat Sen University of Medical Sciences, Guangzhou 510089 P.R.China E mail:zhenglk @ gzsums.edu.cn;Using the method of two color fluorescence in situ hybridization to detect aneuploidy of human sperms[J];CHINESE JOURNAL OF MEDICAL GENETICS;1999-02
3 LIUShengqin ,ZHENGLkang ,DENGLixia ,etal.Departmentof OccupationalHealth ,SchoolofPublicHealth ,SunYat SenUniversityofMedicalSciences,Guangzhou ,5 10 0 89, China;Detection of numerical chromosome aberrations in sperm of workers exposed to benzene series by two color fluorescence in situ hybridization[J];CHINESE JOURNAL OF PREVENTIVE MEDICINE;2000-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LI Xin+1, ZHENG Lu-kang+2, DENG Li-xia+2, ZHANG Qiao+2(1. Lab. Of Genetic Toxicology, Center for Disease Control and Prevention of Guangdong Province, Guangzhou 510300, China; 2. Lab. Of Genetic Toxicology, School of Public Health, Sun Yat-Sen University;DETECTION OF ANEUPLOIDIES FOR CHROMOSOME 9,18 IN SPERM OF NORMAL MALES USING TWO-COLOR FLUORESCENCE IN SITU HYBRIDIZATION[J];Carcinogenesis,Teratogenesis and Mutagenesis;2002-02
2 LONG Hua(Laboratory of Animal Molecular Cytogenetics, Bioscience College of Wuhan University, Wuhan, Hubei430072, China;Key Open Laboratory of Freshwater Fish Germplasm Resources & Biotechnology,Ministry of Agriculture,Yangtze River Fisheries Research Institute of Chinese Academy of Fisheries Sciences,Jingzhou, Hubei 434000, China; Bioscience Collegeg of Shanghai Fisheries University, Shanghai 200090, China)FU Yuan-shuai (Bioscience Collegeg of Shanghai Fisheries University,Shanghai 200090,China)CHEN Ji, ZHAO Gang, LIU Jiang-dong(Laboratory of Animal Molecular Cytogenetics, Bioscience College of WuhanUniversity, Wuhan, Hubei 430072,China)CHEN Jian-wu(Key Open Laboratory of Freshwater Fish Germplasm Resources & Biotechnology,Ministry of Agriculture,Yangtze River Fisheries Research Institute of Chinese Academy of Fisheries Sciences, Jingzhou, Hubei 434000, China)YU Qi-xin (Laboratory of Animal Molecular Cytogenetics, Bioscience College of Wuhan University, Wuhan, Hubei 430072,China);New Methods and New Progress of Chromosomes Researches[J];Journal of Yangtze University(Natural Science Edition) Agri Sci V;2006-01
3 ZHUANG Xin-jie,LU Yang-qing,ZHANG Ming,LU Ke-huan(Animal Reproduction Institute,Guangxi University,Nanning 530005,China);Progress on mammalian sex identification by FISH[J];Journal of Guangxi Agricultural and Biological Science;2008-03
4 Liu Hao, Zhang Changjun, Wang Hua, et alReproductive Medical Center, The People’s Hospital of Shiyan, Shiyan 442000, China;Establishment of An Effective Approach to Dual-color Fluorescence in situ Hybridization of Human Sperm Interphase Nucleus[J];Journal of Hubei Medical University;2005-02
5 LONG Hua Laboratory of Animal Molecular Cytogenetics,Bioscience College of Wuhan University,Wuhan,Hubei430072,China;Key Open Laboratory of Freshwater Fish Germplasm Resources & Biotechnology,Ministry of Agriculture,Yangtze River Fisheries Research Institute of Chinese Academy of Fisheries Sciences,Jingzhou,Hubei 434000,China;Bioscience Collegeg of Shanghai Fisheries University,Shanghai 200090,ChinaFU Yuan-shuai(Bioscience Collegeg of Shanghai Fisheries University,Shanghai 200090,China)CHEN Ji,ZHAO Gang,LIU Jiang-dong Laboratory of Animal Molecular Cytogenetics,Bioscience College of WuhanUniversity,Wuhan,Hubei 430072,ChinaCHEN Jian-wu Key Open Laboratory of Freshwater Fish Germplasm Resources & Biotechnology,Ministry of Agriculture,Yangtze River Fisheries Research Institute of Chinese Academy of Fisheries Sciences,Jingzhou,Hubei 434000,ChinaYU Qi-xin(Laboratory of Animal Molecular Cytogenetics,Bioscience College of Wuhan University,Wuhan,Hubei 430072,China);New Methods and New Progress of Chromosomes Researches[J];Journal of Yangtze University(Natural Science Edition);2006-02
6 Lv Jing,Li Lianbin,Li Xinsheng,et al.Chongqing Institute of Science and Technology for Population and Family Planning,Chongqing 400020;Exploration on the Relationship between Infertility and Y Chromosome Microdeletion as well as Spermatozoa Chromosome Aneuploid[J];Chinese Journal of Family Planning;2009-03
7 Qun Fang ZHANG, Guang Xiu LU (Laboratory of Human Reproductive Engineering, Xiangya Medical School, Central South University, Changsha,Hunan 410078,China);Chromosomal Analysis of Human Sperm Using Multiple Fluorescence in situ Hybridization[J];Acto andrologica Sinica;2002-01
8 SONG Bo 1, CAI Zhi-ming 1, LI Xin 2, DENG Li-xia 3, ZHENG Lü-kang 3 1.Department of Reproductive Surgery, Shenzhen Hospital of Peking University, Shenzhen, Guangdong 518036, China; 2.Center of Disease Prevention and Control of Guangdong Province, Guangzhou, Guangdong 510071, China; 3.Laborary of Genetic Toxicology, School of Public Health, Sun Yat-Sen University, Guangzhou, Guangdong 510089, China;Effect of Benzene on Sperm DNA[J];Nationlal Journal of Andrology;2005-01
9 LI Lian-bing1, XIA Yan-kai2, LI Xin-sheng1, L Jing1, MA Ming-fu1, SONG Ling2, JI Yuan1, YANG Ji-gao1, ZHANG Tian-feng1, CHEN Xiao-xing1, RONG Ying1, ZHAO Le-tian11.Chongqing Institute of Science and Technology for National Population and Family Planning, Chongqing 400020, China; 2.Institute of Toxicology, Nanjing Medical University, Nanjing, Jiangsu 210029, China;An Analysis on Chromosome X,Y and 18 in the Spermatozoa of Asthenospermia Patients by Triple-color Fluorescence in Situ Hybridization[J];National Journal of Andrology;2008-03
10 ZHU Qian-xi1,HUANG Yu-feng2 1.Shanghai Research Institute of Planned Parenthood,Shanghai 200032,China;2.PLA Research Institute of Clinical Laboratory Medicine,Nanjing General Hospital of Nanjing Military Region,Nanjing,Jiangsu 210002,China;Impact of endocrine disrupting chemicals on semen quality[J];National Journal of Andrology;2011-10
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 JI Fu-Yun YU Qi-Xing **- GUO Yi-Qing HAI Yan\ ZHOU Rong-Jia\ LIU Li (College of Life Science, Wuhan University, Wuhan 430072, China);MICROISOLATION AND CHARACTERIZATION OF SINGLE CHROMOSOME IN RICE FIELD EELS (MONOPTERUS ALBUS)[J];Acta Zoologica Sinica;2002-01
2 WU Li-xian (Animal and Poultry Improvement Station of Wenshan Prefecture of Yunnan Province,Wenshan,Yunnan 663000,China);Techniques of Improving Conception Rate of AI in Bovine[J];China Cattle Science;2006-06
3 ;Mechanism of Sex Determination[J];China Comtemporary Medicine;2001-02
4 CAI Ke jun 1,2 ,SI Wei 1,2 ,LI Ya hui 1,2,3 ,JI Wei zhi 1,4 (1.Kunming Institute of Zoology,the Chinese Academy of Sciences,Kunming 650223,China; 2.Graduate School,the Chinese Academy of Sciences,Beijing 100039,China; 3.College of Food Science and Technology,Yunnan Agricultural University,Kunming 650201,China);Evaluation of Mammalian Semen Quality by Flow Cytometry[J];Zoological Research;2003-04
5 WANG Li-yun~1,LV Shan-chao~2,WANG Jin-yu~1(1.Department of Animal Science,Yangzhou Agricultural University,Yangzhou 225009,China;2.Department of Animal Science,Yunnan Agricultural University,Kunming 650201,China);The Mechanism of Sex Determination in Mammals[J];China Animal Husbandry & Veterinary Medicine;2006-10
6 Liu Hao, Zhang Changjun, Wang Hua, et alReproductive Medical Center, The People’s Hospital of Shiyan, Shiyan 442000, China;Establishment of An Effective Approach to Dual-color Fluorescence in situ Hybridization of Human Sperm Interphase Nucleus[J];Journal of Hubei Medical University;2005-02
7 SUN Shu-chun~(1,2),SANG Run-zi~(1,2),SUN Guo-qing~(1,2),LI Dian-hua~3,ZHU Wei-sheng~3,LIWei-jun~3,ZHAO Zhu-jun~(1,2) (1.Animal Science and Technology College of the Agriculture University of Hebei. 2.The Research Centre of Cattle and Sheep Embryonic Technique of Hebei,Baoding of Hebei Province,071001. 3.The Dairy Cattle Farm of HanDan FuYang Milk Industry Ltd.,HanDan of Hebei Province,056000);Effects of Artificial Insemination with Sexing Semen on Pregnant Rate and Female Calf Rate of Dairy Cattle[J];Heilongjiang Journal of Animal Reproduction;2007-01
8 Luo Cheng Hao CaiYao Huan Qiong Ling el.(Dept.of Animal Husbandry, Dept.of Agricultural Biology);A TRIAL OF SEXING SPERM FOR SEXING CONTROL IN DAIRY CATTLE[J];Journal of South China Agricultural University;1992-04
9 WANG Yong-mei 1* ,CUI Ying-xia+1, YIN Hong-lin+2, HUANG Yu-feng+1(1^Central Laboratory of Medicine, Nanjing General Hospital of PLA;2^Department of Pathology,Nanjing General Hospital of PLA,Nanjing 210002,China) ;;MITOCHONDRIAL DNA MUTATION OF SPERMATOZOA OF INFERTILE MALE AND CHANGE OF MITOCHONDRIA-ULTRASTRUCTURE[J];Acta Anatomica Sinica;2001-02
10 Ning ZHANG, Li ZHANG, Xiao-Ping DING, Hai-Ou LU, Jie TANG (Reproduction Centre, 202 Hospital of PLA, Shenyang, Liaoning 110003, China);Analysis of Correlation Between Parameters of Sperm Chromatin Structure Assay and Semen Routine Analysis[J];Acto andrologica Sinica;2003-03
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 HUANG Mei,SHI Hong(Department of Obstetrics and Gynecology,the First Affiliated Hospital of Dalian Medical University,Dalian 116021,China);Progress of abnormal chromosomal detection of human sperm using fluorescence in situ hybridization[J];Journal of Dalian Medical University;2010-03
2 XU Zhi-peng, SUN Hai-xiang, HU Ya-li, ZHANG Ning-yuan, ZHAO XiaCenter of Reproductive Medicine, Drum Tower Hospital Affiliated to Nanjing University Medical College, Nanjing, Jiangsu 210008, China;Laser-assisted Immobilization Causes No Firect Damage to Sperm DNA[J];National Journal of Andrology;2007-03
3 XU Zhi-peng, SUN Hai-xiang, ZHANG Ning-yuanCenter of Reproductive Medicine, Drum Tower Hospital Affiliated to Nanjing University School of Medicine, Nanjing, Jiangsu 210008, China;Sperm DNA Damage and Assisted Reproductive Technology[J];National Journal of Andrology;2008-03
4 CAI Jing,LIANG Pei-yan,YIN Biao,LIANG Jin-ming,LIN Qi,ZENG Yong Institute of Reproduction and Genetics,Shenzhen Zhongshan Urological Hospital,Shenzhen,Guangdong 518000,China;Determination of Sperm DNA Integrity by Improved Sperm Chromatin Structure Assay Test[J];National Journal of Andrology;2009-12
5 WANG Wei,LIANG Guang-tie,PENG Ya-ya,LIU Da-yu,ZHOU Xiao-mianDepartment of Clinical Laboratory,The First People's Hospital of Guangzhou Affiliated to Guangzhou Medical College,Guangzhou,Guangdong 510180,China;Effects of a microfluidic sperm sorter on sperm routine parameters and DNA integrity[J];National Journal of Andrology;2011-04
6 Jin Baofang, Huang Yufeng, Xia Xinyi, Wang Xiulai Andrology Department, General Hospital of Nanjing Command, PLA, Nanjing 210002, China Yang Xiaoyu Center of Clinical Reproductive Medicine, Jiangsu Province Hospital Zhou Zuomin Key Laboratory of Reprodutive Medicine of Jiangsu Province;Effect of Yangjing capsule and Xinxibao on sperm DNA integrity of patients with male infertility[J];Chinese Journal of Andrology;2006-12
7 BO Xin,LI Jing-xia(Normal College of Beijing Union University,Beijing 100011,China);Primary studies on the changes of some nutrient components during fermenta- tion process of Tibetan Kefir yoghurt fermentation process[J];China Dairy Industry;2008-11
8 BO Xin,LI Jing-xia(Normal College of Beijing Union University,Beijing 100011,China);Experiments on Changes of some vitamins and acetaldehyde in fermentative process of the tibetan kefir fermentation milk[J];China Dairy Industry;2009-03
9 BO Xin,LI Jing-xia(Normal College of Beijing Union University,Beijing 100011,China);Experiments on health care to digestive system of mice by the tibetan Kefir fermentation Milk[J];China Dairy Industry;2010-07
10 BO Xin,LI Jing-xia(Normal College of Beijing Union University,Beijing 100011,China);Study on immunity effected by the Tibetan kefir fermentation milk[J];China Dairy Industry;2011-06
【Secondary Citations】
Chinese Journal Full-text Database 5 Hits
1 Zheng Lukang, Deng Lixia, Zhang qiao Lab. of Genetic Toxicology, School of Public Health, Sun Yat Sen University of Medical Sciences, Guangzhou 510089;STUDY ON THE ANEUPLOIDY FREQUENCY IN HUMAN SPERM CHROMOSOME USING FLUORESCENCE IN SITU HYBRIDIZATION (FISH)[J];CARCINOGENESIS, TERATOGENESIS AND MUTAGENESIS;1998-05
2 ZengNian-hua*,PengGuifu,WuChungang,etal.*InstituteofMilitaryMedicineinGuangzhouCommand,Guangzhou510507;Theapplicationvalueofurinaryt,t┐MAinbiologicalmonitoringofbenzeneexposure[J];CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND DCCUPATIONAL DISEASES;1996-03
3 Zheng Lǚkang,Deng Lixia,Zhang Qiao.Department of Industrial Hygiene and Occupational Diseases,School of Public Health,Sun Yat sen University of Medical Sciences,Guangzhou 510089;The frequency of human sperm aneuploidy for chromosome X in workers exposed to carbon disulfide[J];CHINESE JOURNAL OF INDUSREIAL HYGIENE AND DCCUPATIONAL DISEASES;1998-04
4 ZHENG Lukang *, LIU Shengqin, DENG Lixia, ZHANG Qiao.*Laboratory of Genetic Toxicology, Sun Yat Sen University of Medical Sciences, Guangzhou 510089 P.R.China E mail:zhenglk @ gzsums.edu.cn;Using the method of two color fluorescence in situ hybridization to detect aneuploidy of human sperms[J];CHINESE JOURNAL OF MEDICAL GENETICS;1999-02
5 LIUShengqin ,ZHENGLkang ,DENGLixia ,etal.Departmentof OccupationalHealth ,SchoolofPublicHealth ,SunYat SenUniversityofMedicalSciences,Guangzhou ,5 10 0 89, China;Detection of numerical chromosome aberrations in sperm of workers exposed to benzene series by two color fluorescence in situ hybridization[J];CHINESE JOURNAL OF PREVENTIVE MEDICINE;2000-01
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