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《Carcinogenesis,Teratogenesis and Mutagenesis》 2004-02
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Ionizing Radiation Regulated Expression of HeLa Cells by Lipofectamin-Mediated TNFα Gene

LIU Jian_xiang,SU Xu (National Institute for Radiological Protection,China CDC,Beijing100088,China)  
BACKGROUNDAIM: To investigate the expression of p-Egr-TNFα,cell cycle and apoptosis in HeLa cells,which were transfected by recombined plasmid p-Egr-TNFα,induced by 60 Coγ-rays irradiation in different doses. MATERIAL AND METHODS: The expression of TNFαwas detected by ELISA.The cell cycle and apoptosis were detected by Flow Cytometry. RESULTS: Afterγ-rays irradiation in different doses,the expression of TNFαwas(250~400)pg/ml,significantly higher than that of the control group.The percentages of S and G 2 M phase were markedly higher than that of the control group,and showed a dose-dependent increase,the rate of G 0 /G 1 showed a dose-dependent decrease.Cell apoptosis rate showed a dose-dependent increase. CONCLUSION: γ-rays can regulate expression of p-Egr-TNFαby Lipofectamin-mediated TNFαgene in HeLa cells.The exˉpression level shows a significantly increase in transfected HeLa cells.The change of cell cycle and apoptosis shows a dose-dependent effect.
【CateGory Index】: R73-36
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【References】
Chinese Journal Full-text Database 1 Hits
1 LIU Jian-xiang, RUAN Jian-lei, SU Xu, et al. National Institute for Radiological Protection, China CDC, Beijing 100088, China;Overexpression of TNFα gene in SMMC-7721 cells irradiated with ~(12)C~(6+) ion[J];Chinese Journal of Radiological Medicine and Protection;2006-03
【Citations】
Chinese Journal Full-text Database 1 Hits
1 Lu Xing, Xing Ruiyun , Sun Zhixian et al ( Beijing Institute of Radiation Medicine , Beijing 100850);Cloning of the Regulatory Sequence of Mouse Egr-1 Gene and Characteristics of Its Radiation-Inducible Property[J];Chinese Journal of Cancer Biotherapy;1998-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 WU Cong_mei 1, LI Xiu_yi 2(1. Department of Biology, Shantou University Medical college, Shantou 515031 , China; 2. MH Radiobiology Research Unit, School of Preventive Medicine, Jilin University, Changchun 130021 , China);STUDY ON CONSTRUCTION OF pcEgr-IFNγ AND EXPRESSION PROPERTY OF X-RAY INDUCTION IN VITRO[J];Carcinogenesis,Teratogenesis and Mutagenesis;2003-01
2 DU Nan, PEI Xue-tao, LUO Cheng-ji, LI Liang, ZHOU Zhong-min, FENG Kai, BAI Ci-xian, SUN Bin (Department of Radiation Medicine, Third Military Medical University, Chongqing 400038,China);Study of the expression of hematopoietic growth factors regulated with Egr-1 promotor sequence[J];ACTA ACADEMIAE MEDICINE MILITARIS TERTIAE;2000-04
3 WANG Fu you, GAO Jing sheng. Research Institute of Combined Injury, Third Military Medical University, Chongqing 400038, China;The advance in gene-radiotherapy of tumor mediated by the Egr-1 promoter[J];Chinese Journal of Disease Control and Prevention;2004-05
4 Du Nan, Pei Xuetao, Luo Chengji et al. The 304th Hospital of PLA,Beijing 100039;STUDIES ON THE EXPRESSION OF GM-CSF REGULATED BY RADIO INDUCIBLE PROMOTER[J];Medical Journal of Chinese People's Liberation Army;2001-05
5 L Xing, XING Rui Yun, SUN Zhi Xian, PEI Xue Tao, WU Zu Ze(Institute of Radiation Medicine, Beijing 100850,China).;Identification of Human OSM cDNA Integrated into Genome of Mouse Melanoma Cells and Its mRNA Transcripts by PCR Methods.[J];PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS;1999-05
6 XU Jian-hua,YANG Zhan-shan (Nuclear Medicine School,Suzhou University,Jiangsu Suzhou 215007,China);Advances in the Research of the Role of Egr-1 Gene in cells' Respo nse to Ionizing Radiation[J];Chinese Journal of Hemorheology;2002-01
7 ZHU Yi, XIAO Jian-yun, ZHAO Su-ping, et al. (Department of otorhinolaryngology, The first Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China);Effect of ionizing radiation on Egr-1 promoter in NPC cells[J];Chinese Jounal of Otorhinolaryngology-skull Base Surgery;2002-04
8 DU Nan,PEI Xuetao,LUO Chengji, et al. the Third Mititary Medical University,Chongqing 400038,China\;;Studies on the expression of hematopoietic growth factors regulated by Egr-1 promoter \[J];CHINESE JOURNAL OF RODIATION MEDIATION AND PROTECTION;2000-05
9 LIU Jian-xiang, RUAN Jian-lei, SU Xu, et al. National Institute for Radiological Protection, China CDC, Beijing 100088, China;Overexpression of TNFα gene in SMMC-7721 cells irradiated with ~(12)C~(6+) ion[J];Chinese Journal of Radiological Medicine and Protection;2006-03
10 L*a Xing, XING Ruiyun, LU Ping, SUN Zhixian, PEI Xuetao, WU Zuze (Institute of Radiation Medicine, Beijing 100850);Oncostatin M Shows Inhibitory Role and Effect of Gene-Radiotherapy on Melanoma Growth in vivo[J];Chinese Journal of Cancer Biotherapy;2001-01
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 GUAN Xiao-ju, et al (Institute of Immunology, Third Military Medical University, Chongqing 400038, China);CELLULAR EFFECTS OF CYCLOPHOSPHAMIDE ON MOUSE EMBRYOS FROM WHOLE EMBRYO CULTURE SYSTEM[J];CARCINOGENESIS, TERATOGENESIS AND MUTAGENESIS;2000-03
2 Song Yong Mei, Tong Tong, Fu Ming, Dong Li Jia, Jin Shun Qian, Wu Min, Zhan Qi Min National Key Laboratory of Molecular Oncology, Cancer Institute (Hospital), Chinese Academy of Medical Science, Peking Union Medical College, Beijing,100021, P.R.China;Gadd45 Mediated G_2/M Cell Cycle Arrest Induced by BRCA1[J];Chinese Journal of Cancer;2004-05
3 Zhang Junjun Zou Guolin Ou Rong (College of Life Sciences, Wuhan University, Wuhan 430072, China);Progress in Mechanism Studies on the Interaction of(phen) 2Cu + and DNA[J];AMINO ACIDS & BIOTIC RESOURCES;1998-03
4 ZENG Hui- hui1, LIU Jing- sheng2 (1.School of Pharmaceutical Sciences,Peking University, Beijing 100083,China; 2. Department of Chemistry,Baoding Teachers college,Baoding 071051,China);Chemistry of free radical oxidative damage to DNA[J];Journal of Baoding Teachers College;2000-04
5 Ma Yiquan (The 1st Hospital in Huzhou, Huzhou 313000);The Effect of Bolus of Six Drugs Including Rehmannia on Glucorticoid Recepter (GCR) Lever in the Nephrolic Syndrome (NS) Patients' Blood[J];JOURNAL OF ZHEJIANG COLLEGE OF TCM;2000-03
6 HU Bing,AN Hong-mei,SHI Yun-feng,et al (Shanghai university of Traditional Chinese Medicine,Longhua Hospital,Shanghai,200032,China);Influence on Prescription of Invigorate Kidney and Essence Replenishment to Wistar Institute 38 Cell Cycle and Expression of Related Gene[J];Journal of Chengdu University of Traditional Chinese Medicine;2007-02
7 Shuai Xiaorong XiaQingyou Zhu Yong (The Key Sericultural Laboratory of Agriculrural Ministry,College of Sericulture & biotechnology Southwest Agricultural Univorsity ,Chongqing, 400716);Progress and Application of Quantitative PCR[J];Newsletter of Sericultural Science;2002-04
8 GUO Yang1,2,CHEN Shi-jie1,GUO Wan-zhu2,LI Jing3(1.Sichuan Export and Import Inspection Quarantine Bureau,Chengdu,Sichuan,610041,China;2.Animal Biological Technology Center of Sichuan Agricultural University,Yaan,Sichuan,625014,China;3.Material and Biotechnology College,Chengdu University of Technology,Chengdu,Sichuan,610059,China);Advance in Fluorescent Quantitative PCR and Its Applications[J];Progress in Veterinary Medicine;2009-02
9 Long Xianhui~(1,2) Xu Qinzhi~1 He Xingpeng~2 Sui Jianli~1 An Jing~1 Bai Bei~1 Zhou Pingkun~1(1.Beijing Institute of Radiation Medicine,Beijing,100850;2.Public Health School,Nanhua University,Hengyang,421000);ALTERATIONS OF TRANSCRIPTIONAL PROFILE IN NORMAL HUMAN LYMPHOBLASTOID CELLS BY 5cGy OF γ-RAY EXPOSURE[J];Radiation Protection;2006-02
10 Wen Jianhua Li Jianguo Tian Huancheng Li Yanling Wang Xiaoli Zuo Yahui(Department of Radiation Medicine and Environment Medicine,China Institute for Radiation Protection,Taiyuan,030006);STUDY OF DIFFERENTIAL GENE EXPRESSION IN HUMAN HEPATOCYTE EXPOSED TO 50 cGy γ RAY[J];Radiation Protection;2008-02
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