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《Carcinogenesis,Teratogenesis and Mutagenesis》 2004-03
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Use of the Single Cell Gel Electrophoresis/Comet Assay for Detecting the DNA Damage Effect of Formaldehyde to Mouse Testicular Cell in Vivo and in Vitro

DONG Jie-ying 1 ,LOU Zhe-feng 1 ,ZHENG Zhong 2 ,et al (1.Department of Biology,Wenzhou Medical College,Wenzhou325035,China;2.Class of2000laboratory science,Wenzhou Medical College,Wenzhou325035,China)  
BACKGROUNDAIM: To study the DNA damage effect of formaldehyde to mouse testicular cell in vivo and in vitro. MATERIAL AND METHODS: Mice testicular cell was exposed to6,30,60,120μmol/L dose of formaldehye in vitro,and to0.2,2,20mg/kg dose of formaldehyde five days by intraperitoneal injection.The single cell gel electrophoresis was used to detect the DNA damage. RESULTS: In vitro the DNA migration in group of6μmol/L dose obviously showed the significant damage to testicular cell DNA.30μmol/L group have the most pronounced.But the damaging effect when dose increased,DNA migration decreased by contraries.In the highest dose group(120μmol/L)there was no significance effect comparing to the concurrent negative control(P0.05).In vivo the0.2and2mg/kg groups had the statisticsignificance against the concurrent control(P0.01),esˉpecially the0.2mg/kg group. CONCLUSION: Formaldehyde is genotoxic to mouse testicular cell.DNA strand break is the primary DNA damage in low dose.
【Fund】: 浙江省温州市科技发展计划项目(No.wkw03019)
【CateGory Index】: X503.1
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