Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Carcinogenesis,Teratogenesis and Mutagenesis》 2004-04
Add to Favorite Get Latest Update

The Isolation of Anti-tumor Component of Acanthopanax Gracilistylus

SHAN Bao_en,SI Chong_yang,ZHANG Jin_zhong,et al (Research center,The Fourth Hospital of Hebei Medical University,Shijiazhuang050011,China)  
BACKGROUNDAIM: The anti_tumor active components were isolated from Acanthopanax gracilistylus to identify it's molecular weight and supply the scientific evidence for developing new anti_tumor drugs. MATRAIAL AND METHODS: The effects of the active component on the proliferation of tumor cells was investigated by 3 H_TdR inˉcorporation methods.The active component was isolated2D by chromatography.Its elements and molecular weight were measured By SDS_PAGE and2D electrophoresis.Its chemical nature was analyzed by treatment with pronase E and NaIO 4 . RESULTS: By gel_filtration and SDS_PAGE,an64kDa protein was isolated(G100F),which inhibited the proliferation of MT_2tumor cells.The anti _ tumor active component being not a polysaccharide but a protein was measured by treatment with pronaseE and NaIO 4 .This result was further confirmed by2D electrophoresis. CONˉCLUSION: The anti_tumor active component of Acanthopanax gracilistylus is a64kDa protein.The purification of this component will supply scientific evidence for the development of new anti_tumor drugs and the study of the active component.
【Fund】: 国家自然科学基金(No.30271595);; 国家教委;; 河北省教育厅资助项目(No.2001245)
【CateGory Index】: R284
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 5 Hits
1 LU Tuan-wei1,LIU Meng-yu,LI Shu-hong2,LIU Bing1(1.Department of Medical Genetics,School of Basic Medical Sciences,Jilin University,Changchun 130021,China; 2. Department of Pathogenobiology,School of Basic Medical Sciences,Jilin University,Changchun 130021,China );Antagonistic effects of acanthopanasia and hoelen on mutagenesis induced by cadmium[J];Journal of Jilin University(Medicine Edition);2008-04
2 ZHANG Jing-yan1,WU Zhi-cong2,CHENG Xiao-xu,REN Feng-zhi,ZHANG Xue-xia(1.NCPC New Drug Research and Development Co.,Ltd,Shijiazhuang 050001,China;2.The First Hospital of Shijiazhuang,Shijiazhuang 050011,China);Progress on Acanthopanax gracilistylus[J];Hebei Chemical Industry;2013-02
3 LIU Hua-gang,LIANG Qiu-yun,HUANG Hui-xue(Cuangxi Medical University,Nanning,Guangxi,530021,China);The Development of Researches on the Natural Antineoplastic[J];Lishizhen Medicine and Materia Medica Research;2007-08
4 Shen Lihong,Wang Shengli,Zhu Tingchun,Li Ya(Department of Chemistry,Handan College,Handan Hebei 056005);Research and Application of Anti-Tumor Activity Components of Plants[J];Chinese Agricultural Science Bulletin;2010-08
5 SHAN Bao-en, FU Xiao-mei, HUA Zheng-xiang,et al Research Center, The Fourth Hospital of Hebei Medical University, Shijiazhuang (050011);Study on Mechanism of the Anti-tumor Activity of Acanthopanax Gracilistylus[J];Chinese Journal of Integrated Traditional and Western Medicine;2005-09
【Citations】
Chinese Journal Full-text Database 4 Hits
1 CAO Zhi quan Beijing University of Traditional Chinese Medicine;New Thinking About Study of Pharmacodynamic Material Basis and Functional Mechanism in Chinese Materia Medica —Study on the Relation between Morphology and Biological Activity of Chemical Species in Chinese Mate[J];ACTA UNIVERSITATIS TRADITIONIS MEDICALIS SINENSIS PHARMACOLOGIAEQUE SHANGHAI;2000-01
2 XU Song lin, XU Shi min, GAN Ai hua (National Engineering Research Center for Distillation Technology, Tianjin University, Tianjin 300072, China);Application of molecular distillation on separation of natural products[J];Chinese Traditional and Herbal Drugs;2001-06
3 CAI Zhongde; JIANG Tingliang(Institute of Chinese Materia Medica, China Academy of Traditional ChineseMedicine , Beijing 100700 );Discussion on Strengthening the Patent Protection in the Domain of Chinese Materia Medica[J];CHINA PHARMACY;1999-01
4 LU¨ Yang Cheng, LUO Guang Sheng *, DAI You Yuan (Dept. of Chemical Engineering, Tsinghua University, Beijing 100084)$$$$;Development of Extraction Technology in Chinese Medicine[J];Chinese Journal of Pharmaceuticals;2001-05
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 QU Xin-hua(Experimental Middle School of Hengyang in Hunan,Hengyang,Hunan 421001);Extraction and Separation Technique of Essential Oil from Plants[J];Journal of Anhui Agricultural Sciences;2007-32
2 WANG Jun-jie et al(College of Material Sciences and Chemical Engineering,Hainan University,Danzhou,Hainan 571737);Research Progress on Extraction and Detection and Medicinal Value of Anthrone Compounds in Aloe[J];Journal of Anhui Agricultural Sciences;2010-21
3 WANG Yu-hong ,YUAN Gui-ying (Department of Food Science,Henan Vocational College of Agricalture,Zhongmou 451450, China);Extraction and Application of Glycosides in Cornus[J];Storage & Process;2009-06
4 ZHANG Jia-lan (College of Animal Science,Yangtze University,Jingzhou,Hubei 434025,China);Study on Extraction of Polysaccharide from Jerusalem artichoke by Ultrasonic[J];;2008-01
5 LI Jian-xi,YANG Zhi-qiang,WANG Xue-zhi(Lanzhou Institute of Animal Sciences and Veterinary Pharmaceutics,CAAS,Lanzhou,Gansu,730050,China);The Advance and Future of Traditional Chinese Veterinary Medicine[J];Progress In Veterinary Medicine;2005-02
6 ;On Infringement Determination For The Patent Right Of Chinese Medicine Prescription[J];Law Science Magazine;2010-08
7 DONG Li 1,2 , XING Jun 1, WU Cai ying 1* (1. Department of Chemistry, Wuhan University.,Wuhan,430072; 2. Department of Basic Medicine, Xinxiang Medical College,Xingxiang,453003);The Progress in Analytical Methods of Flavor and Essence[J];Journal of Analytical Science;2003-02
8 Tu Jie~(1,2) Zhang Xin- shen~1 Luo Xia~3 Xiao Xuebin~2 Luo Yajun~1 Yuan Dong~(1,4) Li Xiang~1 Zou Yuquan~1 1.College of Light Industry,Textile & Food Engineering,Sichuan University,Chengdu,610065; 2.Chengdu Grain Storage Research Institute,State Grain Administration,Chengdu,610031; 3.Sichuan Institute of Chinese Medicine,Chengdu,610041; 4.Department of Material and Chemical Engineering,Sichuan Institute of Technology,Zigong,643000;Study on the chemical speciation of trace elements in Thlaspi arvense Linn.[J];Analytical Instrumentation;2006-04
9 SHANG Rui1,2,ZENG Hui-cai1,HaoJia2,3(1.State Key Biotechnology Laboratory for Tropical Crops,CATAS,Haikou 571101,China;2.College of Environment and Plant Protection,Hainan Uninversity,Danzhou 571737,China;3 Analysis and Testing Center,CATAS,Haikou,571101,China);Progress on the research in the agricultural antibiotics s eparation and purification[J];Guangdong Agricultural Sciences;2008-02
10 MA Xue-song,TAN Wei,ZHU Qi-xin(School of Chemical Engineering & Technology,Tianjin University,Tianjin,300072,China);Experimental Study on Filtration Separation technology of the Preparation of extraction Solution of Chinese Traditional Medicine[J];Journal of Filter & Separator;2005-04
China Proceedings of conference Full-text Database 3 Hits
1 Wei Qiang, Xu Shimin , Bai Dongmei , Han Zhenzuei (Department of Biochemical Engineering, School of Chemical & Technology; National Engineering Research Center for Distillation Technology, Tianjin University, Tianjin 300072);Study on Purification of Lactic Acid[A];[C];2002
2 Wang Shuhua, Xu Songlin (National Engineering Research Center for Distillation Technology, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072);Technology of Supercritical Fluid Extraction in Combination with Distillation and Application in Extraction of Nature Product[A];[C];2003
3 Wang Huadong,Huang Junyong,Wei Yingfang~* (Cheng du University of TCM The Ministry of Education Key Laboratory of Standardization of Chinese crude drug,Sichuan Chengdu 611137,China);The status quo of the GAP base construction of Chinese pharmacy[A];[C];2010
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Chun_ling, HU Jun_feng, QU Jiang_bin, et al.(School of Public Health, Shandong University, Jinan 250012 , Ch ina);STUDY ON ANTIMUTAGENICITY OF CHINESE HERBAL MEDICINE AND GREEN TEA TO BENZOAPYRENE AND NNK[J];Carcinogenesis,Teratogenesis and Mutagenesis;2003-02
2 SHAN Bao-en 1 ,LIANG Wen-jie 1 ,REN Feng-zhi 2 ,et al (Research Center,The Fourth Hospital of Hebei Medical University,Shijiazhuang050011,China;2.NCPC New Drug R&D co.Ltd,Shijiazhuang050015,China);Study about Anti-tumor Effect of Rhizoma Menispermi Extracts in Vitro[J];Carcinogenesis,Teratogenesis and Mutagenesis;2004-05
3 LI Yan et al(Key Lab of West Biology Recourses and Modern Biotechnology,Education Ministry,College of Life Science of Northwest University,Xian,Shaanxi 710069);Research Progress on the Pharmacological Effects and Synthesis Pathway of Resveratrol[J];Journal of Anhui Agricultural Sciences;2009-11
4 JIANG Chao, QING Chen, MENG Ling hua, et al. Shanghai Institute of Material Medical,Chinese Academy of Sciences,Shanghai 200031,P.R.China;Inhibitory effects of China ganoderma lucid essence (CGLE) on DNA topoisomerases and its ability to induce apoptosis of K562 cells[J];CHINESE JOURNAL OF CANCER;1999-06
5 ZHOU Jun min, CAO Jian guo, LIAO Duan fang Department of Pharmacology, Hengyang Medical College, Hengyang 421001, P R China;Effects of etoposide on growth proliferation and apoptosis in human HIC/S cells[J];CHINESE JOURNAL OF CANCER;2000-04
6 MENG Ling hua, JIANG Chao, DING Jian*, et al Shanghai Institute of Meteria Medical, Chinese Academy of Sciences, Shanghai 200031, P R China;Inhibition of DNA topoisomerases and direct DNA breakage by extract of spirulina plantensis geitl[J];CHINESE JOURNAL OF CANCER;2000-08
7 ZENG Xiao li*, TU Zhi guangDepartment of Clinical Biochemistry in Chongqing University of Medical Sciences, Chongqing 400016, P R China;Total saponins of panax ginseng reverse some plasmo phenotypesin human hepatoma cell line[J];CHINESE JOURNAL OF CANCER;2000-08
8 WANG Tao1*, JIN Ge2, WANG Shu mei1, et al 1 Department of Respiratory Medicine, the First Affiliated Hospital, Henan Medical University, Zhengzhou 450052, P R China 2 The School of Basic Medical Sciences, Henan Medical University, Zhengzhou 450052,;Induced differentiation of human lung adenocarcinoma cells by cinnamic acid[J];CHINESE JOURNAL OF CANCER;2000-08
9 TU Shui ping* , JIANG Shi hu, QIAO Min min, WANG Li fu, ZHANG Shu, YUAN Yao zong, WU Yun li,WU Yu xin. Department of Gastroenterology, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025,P.R.China;Induction of apoptosis by trichosanthin in gastric cancer cell MKN-45[J];Chinese Journal of Cancer;2000-12
10 Jiang Zhong Qing, Zhu Feng Chuan, Qu Jun Ying, Zheng Xiu, You Cai LingDepartment of Obstetrics andGynecology,The Affiliated FirstHospital,Fujian Medical University,Fuzhou,Fujian,350005,P.R.China;Relationship between Expression of Matrix Metalloproteinase (MMP-9)and Tumor Angiogenesis,Cancer Cell Proliferation,Invasion,and Metastasis in Invasive Carcinoma of Cervix[J];Chinese Journal of Cancer;2003-02
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 LUO Ya-nan et al(Qiannan Medical College for Nationalities,Duyun,Guizhou 558000);Analysis on Volatile Components in Cortex Acanthopanacis by GC/MS[J];Journal of Anhui Agricultural Sciences;2010-17
2 Du Ning(Jiangsu Institute for Food and Drug Control,Nanjing 210008,China);Determination of Sodium Demethylcantharidate Injection and its Related Substances by HPLC[J];Journal of North Pharmacy;2012-01
3 WU Fei,YU Shengnan,GE Xijuan,LI Liang,WANG Peng(College of Food Sciences,Northeast Agricultural University,Harbin 150030,China);Research on anti-cancer activities of soybean protein hydrolysates[J];Journal of Northeast Agricultural University;2010-12
4 WANG Zhi-hong1,XUE Jian-bin2,JIANG Wen-yan2,ZHANG Gui-rong2(1.Basic Medical College,Changchun University of Chinese Medicine,Changchun 130117,China; 2.Life Science College,Jilin University,Changchun 130012,China);A study on effects of Menispermum dauricum alkali polysaccharide on Hela cell proliferation[J];Journal of Northeast Normal University(Natural Science Edition);2011-04
5 Han Limei,Wang Xiaofeng,Sun Na,Zhang Jinlu,Gu Yongsheng,Kong Yanfei;Effect of Chinese herbal compounds on chicken infected by MDV[J];Feed Industry;2011-11
6 QUAN Mei-ping(The college of chemistry and life science,Weinan Teachers University,Weinan 714000,China);Researches on Pharmacologic Effect of Essential Oil in Schizonepeta tenuifolia Briq[J];Modern Food Science and Technology;2013-06
7 LI Yu-dong,DIAO Yong,WANG Li-qiang(Institute of Molecular Medicine Huaqiao University,Engineering Research Certer of Molecular Medicine,Ministry of Education,Quanzhou Fujian 362021,China);The rencent advances of the antitumor effects of paeonia lactiflora pallas and its constituents[J];Strait Pharmaceutical Journal;2009-12
8 WANG Xiao-juan1,2,LIU Yan-ru3,XIAO Bing-kun2,YANG Jian-yun2,YANG Yan1,HUANG Rong-qing2 (Department of Pharmacology,Anhui Medical University1,Hefei 230032,P.R.China;Institute of Radiation Medicine,Academy of Military Medical Sciences2,Beijing 100850,P.R.China;School of Pharmccology Engineering,Shenyang Pharmary University3,Shenyang 110016,P.R.China);Review on Anti-tumor Effects and Mechanisms of Toosendanin[J];Science Technology and Engineering;2011-02
9 LIAO Xia1,LI Cai-hong2,DING Hang2 (1.Department of Biology,Guangdong Medical College,Zhanjiang 524023,China;2.Institute of Biochemistry and Molecular Biology,Guangdong Medical College,Zhanjiang 524023,China);Effect of Berberine hydrochloride from coptis Chinensis franch on the proliferation of three kinds of carcinoma cell lines[J];Journal of Southeast University(Medical Science Edition);2011-02
10 KONG Weixin1,YANG Yongming2,ZOU Jun3,QU Jie3,TIAN Yue2(1.Chengde Medical University,Chengde 067000,Hebei,China;2.Second Hospital of Baoding,Baoding 071000,Hebei,China;3.Affiliated Hospital of Chengde Medical College,Chengde 067000,Hebei,China);Effect of the Pteris Multifida Flavones on the Metastasis Ability of Human Osteosarcoma Cell Line MG-63 Cells[J];Journal of Liaoning University of Traditional Chinese Medicine;2013-01
China Proceedings of conference Full-text Database 2 Hits
1 HAN Jiang-min,LIN Neng-ming ,FANG Luo (Zhejing Cancer Hospital,Hangzhou 310022);Analysis of the Usage of Anti-neoplastic Drugs in Our Hospital During 2002 to 2005[A];[C];2006
2 Li Xiao Ying,Ma Chuan Mei,Zhang Lian Feng (Key Laboratory of Human Diseases Comparative Medicine,Ministry of Health;Institute of Laboratory Animal Science,Chinese Academy of Medical Sciences(CAMS) & Comparative Medicine Centre,Peking Union Medical Collage(PUMC),Beijing 100021,China);The comparison of tumor growth in the implanted mouse model detected by optical imaging system and a slide caliper[A];[C];2009
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Lu Hantao etal.;Aromatic Oils Preparation by Molecular Distillation[J];Guangdong Chemical Industry;1992-02
2 GONG Fan, PENG YuanGui, CUI Hui, LIANG YiZeng (College of Chemistry and Chemical Engineering, Institute of Chemometrics and Chemical Sensing Technology, Hunan University, Changsha, 410082; Institute of Materia Medica, Hunan Academy of Tradition;HELP Applied to Traditional Chinese Medicine Analysis Determination of Components of Cordyceps Sinensis[J];CHEMICAL RESEARCH IN CHINESE UNIVERSITIES;1999-02
3 Qin Wei;Yuan Yonghui;Dai Youyuan(Department of Chemecal Engineering,Tsinghua Unversity,Beijing,100084);Improvement of Separation Processes by Using Ultrasound[J];Chemical Industry and Engineering Progress;1995-01
4 Wu Ming;Ren Zhongjiao;Wang Jie (Department of Chemistry,The South-CentralCollege for Nationalities,Wuhan,Postcode 430074);A NEW TECHNOLOGY FOR EXTRACTION OF NATURE PAPRIKA PIGMENT FROM PAPRIKA[J];Fine Chemicals;1994-06
5 Zhang Yonghong (Department of Pharmacy,Lanzhou Medical College);A Method Selection of Extract Anthraquinone from Chinese Rhubarb[J];JOURNAL OF LANZHOU MEDICAL COLLEGE;1996-02
6 Guo Xiaowu;Zhang Fucheng;Lin Shuyu (Applied Acoustics Institute,Shaanxi Normal Univ., Xi'an 710062);Effect of different ultrasonic frequencies on extracting baicalin[J];Journal of Shaanxi Normal University(Natural Science Edition);1995-01
7 Xie Yang, Wang Zhanyong, Zhang Yonghong, et al Xinjiang Kurle Chinese Medicine Factory, Kurle 841000;Study on Prosessing Glycyrrhizic Acid Inclusion Compound[J];ShiZhen Journal of Traditional Chinese Medicine Research;1998-06
8 WT4”,5BZ]GONG Fan 1, SONG You Qun 1, PENG Yuan Gui 2, LIANG Yi Zeng 1, AKM Leung 3, Foo tim Chau 3 (1. College of Chemistry and Chemical Engineering, Institute of Chemometrics and Chemical Sensing Technology, Hunan University, Changsha 41008;ANALYSIS OF THE VOLATILE OIL OF RHIZOMA ATRACTYLODIS IN PING-WEI POWDER WITH GC/MS[J];ACTA PHARMACEUTICA SINICA;2000-05
9 WT4”,5BZ]Li Xiao ning 1, Cui Hui 1, Song You qun 1, Liang Yi zeng 2 (1. College of Chemistry and Chemical Engineering, Institute of Chemometrics and Chemical Sensing Technology, Hunan University, Changsha 410082, China; 2. College of Chemistr;ANALYSIS OF THE ESSENTIAL OIL OF SCHISANDRA CHINENSIS (TURCZ.)BAILL. WITH GC/MS[J];Acta Pharmaceutica Sinica;2001-03
10 Gong Fan (Gong F), Peng Yuangui (Peng YG) 1, Cui Hui (Cui H), Liang Yizeng (Liang YZ), Alexander K.M.Leung (A.K.M Leung) 2 and Foo tim Chau (F.T. Chau) 2 (College of Chemistry and Chemical Engineering, Institute of Chemometrics and Chemical Sen;HYPHENATED CHROMATOGRAPHY APPLIED TO MULTICOMPONENT DETERMINATION OF CORDYCEPS SINENSIS[J];ACTA PHARMACEUTICA SINICA;1999-03
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved