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《Carcinogenesis,Teratogenesis and Mutagenesis》 2004-04
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The Isolation of Anti-tumor Component of Acanthopanax Gracilistylus

SHAN Bao_en,SI Chong_yang,ZHANG Jin_zhong,et al (Research center,The Fourth Hospital of Hebei Medical University,Shijiazhuang050011,China)  
BACKGROUNDAIM: The anti_tumor active components were isolated from Acanthopanax gracilistylus to identify it's molecular weight and supply the scientific evidence for developing new anti_tumor drugs. MATRAIAL AND METHODS: The effects of the active component on the proliferation of tumor cells was investigated by 3 H_TdR inˉcorporation methods.The active component was isolated2D by chromatography.Its elements and molecular weight were measured By SDS_PAGE and2D electrophoresis.Its chemical nature was analyzed by treatment with pronase E and NaIO 4 . RESULTS: By gel_filtration and SDS_PAGE,an64kDa protein was isolated(G100F),which inhibited the proliferation of MT_2tumor cells.The anti _ tumor active component being not a polysaccharide but a protein was measured by treatment with pronaseE and NaIO 4 .This result was further confirmed by2D electrophoresis. CONˉCLUSION: The anti_tumor active component of Acanthopanax gracilistylus is a64kDa protein.The purification of this component will supply scientific evidence for the development of new anti_tumor drugs and the study of the active component.
【Fund】: 国家自然科学基金(No.30271595);; 国家教委;; 河北省教育厅资助项目(No.2001245)
【CateGory Index】: R284
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