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《Carcinogenesis,Teratogenesis and Mutagenesis》 2004-05
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Detection of DNA Adduct of Nonvolatile Organic Compounds in Tap Water by Using Nuclease P 1 Mediated ~(32)P-postlabeling Method

TANG Fei,GU Kang-ding,YUN Luo-jia,et al (Institute of Environmental Medicine,Tongji Medical College,Huazhong University of Science and Technology,Wuhan430030,China).  
BACKGROUNDAIM: The DNA adducts of the nonvolatile organic compounds(NOCs)in tap water were studied for further validating genotoxicity of NOC S and its mechanism at molecular level. MATERIAL AND METHODS: The sample of tap water derived from Donghu lake of Wuhan was collected and the nonvolatile organic compounds(NOCs)in water were concentrated on XAD_2resin.The DNA adducts of such NOCs were examined by using 32 P_postlabeling method. RESULTS: Some NOCs_DNA adducts could be found in biological samples inˉcluding calf thymus DNA reacted with NOCs and liver of male mice treated with NOCs. CONCLUSION: DNA could be damnified by the NOCs in tap water directly,and the DNA adducts of such NOCs were formed in vitro and in vitro test.
【Fund】: 国家"九五"重点科技攻关计划项目(No.96-911-07-02-02)
【CateGory Index】: R114
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