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《Carcinogenesis,Teratogenesis and Mutagenesis》 2004-05
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Comparison Research of Whole Blood Specimen and Abstracted Lymphocyte in Comet Assay

DUAN Lian,YANG Jing-yu,YU Hai,et al (Department of Pharmacology,Shenyang Pharmaceutical,Shenyang110016,China) AbstractBACKGROUND&AIM: Aim To Compare the difference between using whole blood specimen and abˉstracted lymphocyte in comet assay in order to simplify the process of comet assay. MATERIAL AND METHODS: The whole blood specimen and  
ed lymphocyte of mice after injecting K 2 Cr 2 O 7 and ethanol were used in comet assay to compare the degree of DNA damage. RESULTS: There was no difference of DNA damage of whole blood specimen and abstracted lymphocyte induced by those two noxious substances. CONCLUSION: Whole blood specimen is more convenient than abstracting lymphocytes in comet assay for detecting and screening of medicine on a large scale.
【CateGory Index】: R114
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【References】
Chinese Journal Full-text Database 10 Hits
1 WANG Guang-jun,LIU Zhi-hongAN Xiao-dan,ZHANG Xiao-yu,ZHANG Yong,LIU He-rong,ZHU Ling-qin,MA Xiao-mei(Department of Occupational and Environmental Health,Ningxia Medical University,Yinchuan 750004,China);Cytotoxicity and Genotoxicity of Beryllium Sulfate to Human Embryonic Lung Fibroblast[J];Carcinogenesis,Teratogenesis & Mutagenesis;2009-03
2 DUAN Zhi-kai~1,SHI Ai-li~2,LIU Jian-gong~1, WANG Bin-sheng~2,GUO Wan-long~1,ZHANG Shu-xian~1 (1.Radiation Medicine Institute and Environmental Medicine,China Institute for Radiation Protection,Taiyuan 030006;2.Shanxi Province Rongjun Hospital,Taiyuan 030006,Shanxi,China);High dose radiation-induced DNA damage using single cell gel electrophoresis[J];Carcinogenesis,Teratogenesis & Mutagenesis;2011-06
3 LUO Rong, LIU Zhi-HongDepartment of Labour and Environmental Hygiene, College of Public Health, Ningxia Medical College, Yinchuan 750004, China;Dose-dependent effect of beryllium sulfate on DNA damage in mouse lymphocytes in vitro[J];Journal of the Fourth Military Medical University;2007-09
4 Jiang Yaqi~(1,2) Chen Ying~2 Liu Fenju~1 Li You~(2,3)(1.School of Radiology Medicine and Public Health,Soochow University,Suzhou,215123;2.Beijing Institute of Radiation Medicine,Beijing,100850;3.School of Public Health,Nanhua University,Hunan hengyang,421002);STUDY ON RELATIONSHIPS OF TIME-RESPONSE AND DOSE-RESPONSE OF RADIATION-INDUCED DNA DAMAGES USING SINGLE CELL GEL ELECTROPHORESIS FOR RAPID DOSE ASSESSMENT[J];Radiation Protection;2006-06
5 Xu Haijuan1,Feng Benxiu1,Lin Jian2(1.Guangdong Prevention & Treatment Center for Occupational Diseases,Guangzhou 510310; 2.School of Public Health,Shanxi Medical University,Taiyuan 030001,China);Trace Quantities of Whole Blood on the Experimental Method of the Comet Assay[J];Guangdong Chemical Industry;2011-10
6 LI Su-ping(Department of Physical Education,Hanshan Normal College,Chaozhou 521041,Guangdong China);The Application of the Micro-Whole Blood Specimen in SCGE in Function Evalution[J];Journal of Harbin Institute of Physical Education;2007-01
7 LI Su-ping~1 CHANG Bo~2 1.Department of Physical Education,Hanshan Normal University,Chaozhou 521041,China; 2.Department of Sports Physiology Biochemistry,Shenyang Physical Education Institute,Shenyang 110102,China;The Effect of Ginsenosides of Stem-Leaf on DNA Damage of Lymphocyte in Rats During Overtraining[J];Journal of Hanshan Normal University;2007-03
8 ZHANG Jing- ying,HAN Chun-ji*,LI Lian-ji(Department of Hygiene,Yanbian University College of Medicine,Yanji, 133000,China);Protective Effect of Taurine on DNA Damage Induced by Cadmium Chloride in vitro[J];Journal of Environmental & Occupational Medicine;2006-05
9 SUN Zhen~(1.2) PAN Jing-ju~2 XIE Chao-jun~2 BAO Rong~3 TAN Xiao-dong~(2*) 1.Shanghai Fengxian Center for Disease Control and Prevention,Shanghai 201400,China; 2.School of Public Health,Wuhan University,Wuhan 430071,China; 3.Dept.of Forensic Medicine,School of Medicine,Wuhan University,Wuhan 430071,China;Detection of the DNA Damage of White Blood Cells Induced by Sub-chronic Inhalation of Carbon Disulfide in Mice by Comet Assay[J];Journal of Environmental & Occupational Medicine;2007-04
10 XU Hai-juan,FENG Ben-xiu,YANG-Min.(1.Guangdong Prevention & Treatment Center for Occupational Diseases,Guangzhou 510310 2.Shanghai Institute of Occupational Diseases for Chemical Industry,Shanghai 200041);Trace quantities of whole blood on the experimental method of the comet assay[J];China Tropical Medicine;2011-12
【Citations】
Chinese Journal Full-text Database 2 Hits
1 ZHANG Ai hua,HUANG Xiao xin,LI Jun,et al (Dept.of Preventive Medicine,Guiyang Medical College,Guiyang 550004,China) [;Detection of DNA damage in blood cells of the patients of coal burnt arsenism by SCGE[J];CHINESE JOURANL OF CNDEMIOLOGY;2000-01
2 SUN Shu qiu, JIANG Shu lin, YAO Hong ju, SUN Dian jun (Chinese Center for Endemic Disease Control, Harbin Medical University, Harbin 150086, China);A new approach for directly using micro-whole blood specimen in comet assay and its application in biomonitoring[J];Chinese Journal of Endemiology;2003-02
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LIN Lin,SUN Shu-qiu (Institute of KBD,Harbin Medical University,Harbin 150001, China);Study on DNA damage and repair induced by deoxynivalenol in cultured Vero cell with Comet Assay[J];Chinese Journal of Control of Endemic Disease;2004-03
2 LI Yan-hong,BO Xiao-zhen(Inner Mongolia Center for Endemic Disease Control and Research Huhhot 010020);Review on the Mechanism of Carcinogenesis of Arsenic[J];Chinese Journal of Control of Endemic Diseases;2007-04
3 Yan Xuekun Chen Ying Du Jie Zhang Xueqing(Institute of Radiation Medicine,Academy of Military Medical Sciences,Beijing,100850);APPLICATION OF SINGLE CELL GEL ELECTROPHORESIS ASSAY IN RADIATION BIODOSE ESTIMATION AND ENVIRONMENT MONITORING[J];Radiation Protection;2009-01
4 Xu Haijuan1,Feng Benxiu1,Lin Jian2(1.Guangdong Prevention & Treatment Center for Occupational Diseases,Guangzhou 510310; 2.School of Public Health,Shanxi Medical University,Taiyuan 030001,China);Trace Quantities of Whole Blood on the Experimental Method of the Comet Assay[J];Guangdong Chemical Industry;2011-10
5 CHENG Shu-qun *,ZHOU Yan-rong *,WANG Yi-nan,et al ( *Department to Preventive Medicine,Chongqing Medical University,Ch ongqing 400016 China);Study on Hidden Damage to Animals by Fume Emitted from Coal Burning[J];Industrial Health and Occupational Diseases;2002-05
6 ZHANG Aihua1,CHEN Lang1,ZHANG Ran1,LI Jun1,ZHAO Zhuandi1,ZOU Zhilin1 HUANG Xiaoxin2,ZHANG Bixia2(1.Department of Toxicology,School of Public Health,Guiyang Medical College,Guiyang 550004,Guizhou,China;2.The 44th Hospital of PLA ,Guiyang 550009,Guizhou,China);The Relationship between Hair Trace Elements and Arsenic Poisoning in Arsenism Patient Caused by Coal-burning[J];Journal of Guiyang Medical College;2008-06
7 LI Su-ping(Department of Physical Education,Hanshan Normal College,Chaozhou 521041,Guangdong China);The Application of the Micro-Whole Blood Specimen in SCGE in Function Evalution[J];Journal of Harbin Institute of Physical Education;2007-01
8 ZHANG Qiao-li1,WU Ri-na2(1.Department of Posthraduate 2009,Inner Mongolia Medical College,Huhhot 010059 China;2.Department of Dermatdogist,The Affiliated Hospital of Inner Mongolia Medical College,Huhhot 010059,China);Advances of Pathogenesis of Endemic Arsenic Poisoning[J];Inner Mongolia Medical Journal;2011-09
9 XU Hai-juan,FENG Ben-xiu,YANG-Min.(1.Guangdong Prevention & Treatment Center for Occupational Diseases,Guangzhou 510310 2.Shanghai Institute of Occupational Diseases for Chemical Industry,Shanghai 200041);Trace quantities of whole blood on the experimental method of the comet assay[J];China Tropical Medicine;2011-12
10 Li Su-ping1, Chang Bo21Department of Physical Education, Hanshan Normal University, Chaozhou 521041, Guangdong Province, China; 2Department of Sports Human Science, Shenyang Sports College, Shenyang 110102, Liaoning Province, China;DNA damage to rat lymphocytes assessed by Micro-Whole Blood Specimen in single cell gel electrophoresis during overtraining[J];Journal of Clinical Rehabilitative Tissue Engineering Research;2008-38
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Ma Ai guo, Zang Jin lin, Song Feng rong, Zhang Ri jin (Institute of Human Nutrition, Medical College of Qingdao University, Qingdao 266021, China);COMPARISON RESEARCH OF SCGE, SCE AND CHROMOSOME ABERRATION FOR DETERMINING DNA DAMAGE[J];Carcinogenesis,Teratogenesis and Mutagenesis;2001-03
2 MENG Zi qiang\+1, ZHANG Lian zhen\+2 (1. Institute of Environmental Medicine and Toxicology, Shanxi University, Taiyuan 030006,China; 2. China Institute for Radiation Protection, Taiyuan 030006, China);DNA OXIDANT DAMAGE INDUCED BY TOBACCO SMOKE IN HUMAN BLOOD LYMPHOCYTES[J];Carcinogenesis,Teratogenesis and Mutagenesis;2001-03
3 CAO Yi, CHEN Rui, WANG Zhong_wen, et al(China Institute for Radiation Protection,Taiyuan 030006, China);MEASUREMENT OF DNA DAMAGES INDUCED BY RADIATION AND CHEMICALS USING COMET ASSAY[J];Carcinogenesis,Teratogenesis and Mutagenesis;2003-03
4 SONG Yan,ZHANG Xiao-peng,JIA Xu-dong,ZHANG Wen-zhong,WANG Wei,CUI Wen-ming,ZHANG Xin,LI Ning(National Institute for Nutrition and Food Safety,Chinese Center for Disease Control and Prevention,Beijing 100021);Potential Mechanisms of Oxidative Damage Mechanisms by Smoking[J];Carcinogenesis,Teratogenesis & Mutagenesis;2009-01
5 Ma Aiguo 1,Andrew R.Collins 2,Susan J.Duthie 2 1Department of Human Nutrition,Qingdao Medical College,Qingdao 266021, 2The Rowett Research Institute,U.K.;THE DIFFERENCES OF DNA DAMAGE AND REPAIR BETWEEN SEVERAL TYPES OF CELLS[J];CARCINOGENESIS, TERATOGENESIS AND MUTAGENESIS;1997-03
6 Huang Jiwu 1,Lin Jiancheng 2,McCormick JJ 3 1 Dept.of Public Health, Medical College of Beijing,Beijing 101300, 2Xi'an Medical University, 3Michigan State University;STUDIES OF MULTISTEP PROCESS OF ENVIRONMENTAL CARCINOGENESIS USING IN VITRO MODEL FOR HUMAN FIBROBLAST TRANSFORMATION[J];CARCINOGENESIS, TERATOGENESIS AND MUTAGENESIS;1998-06
7 Meng Ziqiang 1\ and Zhang Lianzhen 2 1\ Division of Environmental Biological Toxicology and Department of Life sciences, Shanxi University, Taiyuan 030006\ 2 China Institute for Radiation Protection, Taiyuan 030006;PRINCIPLE AND METHOD OF THE SINGLE CELL MICROGEL ELECTOPHORESIS (SCGE) TECHNIQUE TO STUDY DNA DAMAGE IN MAMMALIAN CELLS[J];CARCINOGENESIS, TERATOGENESIS AND MUTAGENESIS;1998-06
8 Gui Huali Fu Kecui (Institute of Physical Education,Hubei University,Hubei430062);Effects of Exercise on Body Immune Function and Modulation Mechanics[J];Journal of Anhui Sports Science;2003-01
9 Zhu Yuzhuo, Liu Nianzhi, Xing shenyang, Sun Kai (Department of Medical Genetics,N, Bethune Univ of Med,Sci;Changchun Railway Hospital);Protective effect of Ginsenosidesfrom stem and leaves on damage to mouse genetic materials[J];JOURNAL OF NORMAN BETHUNE UNIVERSITY OF MEDICAL SCIENCE;1994-04
10 CAO Wei-guo~(1,2),LIU Zhi-qin~1,SHAO Yun~1,TAO Yan-duo~*(1 Northwest Institute of Plateau Biology, Chinese Academy of Sciences ,Xining 810001,China;2 Graduate School of the Chinese Academy of Sciences,Beijing 100031,China);A progress in pharmacological research of flavonoids[J];Acta Botanica Boreali-occidentalia Sinica;2003-12
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 WANG Guang-jun,LIU Zhi-hongAN Xiao-dan,ZHANG Xiao-yu,ZHANG Yong,LIU He-rong,ZHU Ling-qin,MA Xiao-mei(Department of Occupational and Environmental Health,Ningxia Medical University,Yinchuan 750004,China);Cytotoxicity and Genotoxicity of Beryllium Sulfate to Human Embryonic Lung Fibroblast[J];Carcinogenesis,Teratogenesis & Mutagenesis;2009-03
2 DUAN Zhi-kai~1,SHI Ai-li~2,LIU Jian-gong~1, WANG Bin-sheng~2,GUO Wan-long~1,ZHANG Shu-xian~1 (1.Radiation Medicine Institute and Environmental Medicine,China Institute for Radiation Protection,Taiyuan 030006;2.Shanxi Province Rongjun Hospital,Taiyuan 030006,Shanxi,China);High dose radiation-induced DNA damage using single cell gel electrophoresis[J];Carcinogenesis,Teratogenesis & Mutagenesis;2011-06
3 Yan Xuekun Chen Ying Du Jie Zhang Xueqing(Institute of Radiation Medicine,Academy of Military Medical Sciences,Beijing,100850);APPLICATION OF SINGLE CELL GEL ELECTROPHORESIS ASSAY IN RADIATION BIODOSE ESTIMATION AND ENVIRONMENT MONITORING[J];Radiation Protection;2009-01
4 YAN Xuekun CHEN Ying DU Jie ZHANG Xueqing LUO Yisheng (Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China);Reasonable threshold value used to segment the individual comet from the comet assay image[J];Journal of Radiation Research and Radiation Processing;2009-01
5 SU Xiao-tian et al(Department of Biotechnology,Liaoning Agricultural College,Yingkou,Liaoning 115009);Determination of Polysaccharides in Ginseng Stem Leaves by Phenol-Sulfuric Acid Method[J];Journal of Anhui Agricultural Sciences;2012-24
6 Rong Qinglin1,Liu Li2,Wang Qin3,Liu Qiang3,Chen Yanfang1,Jiang Wenhua1,Li Xiaodong1(The Second Hospital of Tianjin Medical University:1.Department of Radiation Oncology;2.Tianjin Institute of Urology,Tianjin 300211,China; 3.Key Laboratory of Molecular Nuclear Medicine,Institute of Radiation Medicine,Chinese Academy of Medical Sciences and Peking Union Medical College,Tianjin 300192,China);Preliminary study of relationship between radiation-induced mitochondrial DNA 4 977 bp deletion and radiosensitivity of human tumor cell lines[J];Chongqing Medicine;2012-19
7 Jiang Yuanyuan,Wang Chunhua,Sui Chunsheng,Li Jingshun,Zhao Shuhua;DNA Damage of Bone Marrow Cells in Mice Induced by Nano-Fe_3O_4 Particles[J];Journal of Environmental Hygiene;2012-06
8 JIANG Lin 1,MU Wan-jun 1,LIU Guo-ping 1,XU Yun-shu 1,LUO Shun-zhong 1,GAO Qing-xiang 2 1.Institute of Nuclear Physics and Chemistry,Mianyang 621900,China; 2.School of Live Sciences,Lanzhou University,Lanzhou 730000,China;Application of Single Cell Gel Electrophoresis in Post-Evaluation of Organism Radiation Damage[J];Journal of Nuclear and Radiochemistry;2009-01
9 ZOU Yong,WANG Li-feng,HU Xiang-jun*(Institute of Radiation Medicine,Academy of Military Medical Sciences,Beijing 100850,China);Dose-response relationship of electromagnetic radiation and its research progress[J];Military Medical Sciences;2012-03
10 LI Xiao-wei,GUAN Huai,PIAO Feng-yuan,LI Qiu-juan,XU Lei(Department of Obstetrics and Gynecology,No.210 Hospital of PLA,Dalian 116021,China);DETECTION OF BERYLLIUM,THORIUM,AND URANIUM IN MATERNAL AND UMBILICAL CORD BIOOD[J];Journal of Preventive Medicine of Chinese People's Liberation Army;2010-03
China Proceedings of conference Full-text Database 1 Hits
1 ZHANG Na (Shenyang Sports College,Lliaoning,Shengyng 110102);Researching Status of the Rats Swimming Model Establishing of Exerting Intervention[A];[C];2007
【Secondary Citations】
Chinese Journal Full-text Database 3 Hits
1 Wang Lianfang (Xinjiang Institute for Endemic Disease Control and Research, Urumqi 830002);Studies on Working Criteria of Endemic Arsenism Control[J];ENDEMIC DISEASES BULLETIN (CHINA);1994-01
2 ZHANG Ai hua,HUANG Xiao xin,LI Jun,et al (Dept.of Preventive Medicine,Guiyang Medical College,Guiyang 550004,China) [;Detection of DNA damage in blood cells of the patients of coal burnt arsenism by SCGE[J];CHINESE JOURANL OF CNDEMIOLOGY;2000-01
3 Zhou Yunshu,Zhou Daixing,Zheng Baoshan,et alSouthwest Prefecture Epidemic Prevention Station,Guizhou Province 562400;Epidemiological investigation on coalburning type of arsenic poisoning in different environments within 20 years[J];CHINESE JOURANL OF CNDEMIOLOGY;1998-01
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