Effect on Testicular Germ Cell and Sperm in Mouse After Exposure to Hydroxylammonium Nitrate
JIA Qing-jun, YANG Lu-jun, LIU Jin-yi, et al (Department of Hygienic Toxicology, Preventive Medicine College, Third Millitary Medical University, Chongqing 400038, China)
BACKGROUND AIM: To study the reproductive damage of testicular germ cell and the deformity of sperm in mouse induced by Hydroxylammonium Nitrate (HAN) . MATERIAL AND METHODS: Testicular germ cell micronucleus test, sperm deformity assay and flow cytometry were used. RESULTS: It showed that the percentage of deformity in sperm was markedly increaed as the dose increased(P 0.05), the percentage of micronucleus was significately decreased as the dose increased (P 0.05) . The toxic effect of sperm was correlated with exposuring HAN . CONCLUSION: It suggested that HAN might have reproductive damage effect to testicular germ cell and sperm in mice.