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《Carcinogenesis,Teratogenesis and Mutagenesis》 2004-06
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Studies of tk Gene Mutation by Hydroxylammonium Nitrate

JIA Qing-jun, LIU Sheng-xue, LIU Jin-yi, et al (Department of Hygienic Toxicology, Preventive Medicine College, Third Millitary Medical University, Chongqing 400038, China)  
BACKGROUND AIM: To investigate the effects of Hydroxylammonium Nitrate (HAN) on the tk gene of mouse lymphoma cells. MATERIAL AND METHODS: L5178Y cells were treated with Hydroxylammonium Nitrate with different concentrations, to determine cytotxicity and mutation at different time phases. The numbers of positive wells were counted . The cell plating efficiency, relative suspension growth and mutation frequency were determined. RESULTS: The results showed that HAN (50-500 μg/ ml) induced tk gene mutation with mutation frequency 1 - 19 times higher than that of spontaneous mutation frequency of L5178Y cells and severe cytotoxic responses. CONCLUSION: Hydroxylammonium Nitrate can exert cytotocicity and mutagenic effects on tk geng in L5178Y cells.