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《Carcinogenesis,Teratogenesis & Mutagenesis》 2006-01
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Study of DNA Damage of Lead Acetate on Germ Cells of Mice in Vivo and in Vitro

DONG Jie-ying 1 ,JIN Long-jin 1,ˇ ,LOU Zhe-feng 1 , ZHENG Zhong 2 ,ZHAO Hui-ling 3 (1.Department of Biology,Wenzhou Medical College;Wenzhou 325035,Zhejiang,China;2.Department of Laboratory Medicine, Wenzhou Medical College;3.Centre of Experimental Animal, Wenzhou Medical College,Wenzhou325035,Zhejiang,China)  
BACKGROUNDAIM: To explore the DNA damaging effect of lead acetate on germ cell of mice in vivo and in vitro. MATERIAL AND METHODS: Mouse testicular germ cells were exposed to50,100,500and1000μmol/L lead acetate in vitro,and then exposed to12.5,25,and50mg/kg of lead acetate for five days by intraperitoneal injection.Mouse testicular germ cells were collected on the6th day.Comet assay was used to detect the effects of the damage and electrophoretic mobility distance of DNA. RESULTS: In vitro DNA lesion were detected in all4lead acetate treated groups(compared with negative control P0.01),and was dose-related(visual scoring r=0.5114,P0.01,tail moment r=0.407,P0.01).In vivo DNA damage was detected in3lead acetate treated groups(compared with negative control P0.01),and was also dose-related(visual scoring r=0.4848,P0.01,tail moment r=0.5314,P0.01). CONCLUSION: Lead acetate is genotoxic to mouse testicular germ cells,by inducing mouse testicular germ cell DNA damage.
【Fund】: 浙江省温州市科技发展计划项目(No.S2002A018)
【CateGory Index】: R114
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