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《Carcinogenesis,Teratogenesis & Mutagenesis》 2006-02
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Effects of Lanthanide Ions in Rare Earths on Genomic DNA of E.coli

WANG Cheng-run 1 ,CHEN Hua-bo 1 ,Yang Fan 2 ,GUO Han-qing 2 ,WANG Zi-yao 2 ,DING Tie-lin 2 (1.Department of Chemistry and Biology,Huainan Normal College,Huainan232001,Anhui,China;2.Undergraduates of Year2002,Department of Chemistry and Biology,Huainan Normal College,Huainan232001,Anhui,China)  
BACKGROUND AIM: To investigate genotoxic effect of lanthanide ions on prokaryote by observing the effects of lanthanide nitrate on genomic DNA of E.coli. MATERIALS AND METHODS: TreatmentⅠ:E.coli was cultured in LB medium with different concentrations of lanthanide nitrate(10,40,80,160,320mg/L),LB medium without lanthanide nitrate was the control.Genomic DNA was extracted at10,15,and20h.Then Agarose gel electrophoresis was performed Treat mentⅡ:E.coli was cultwred in LB medium with different concentrations of lanthanide nitrate(50,200,400,800,1600mg/L)LB medium without lanthanide nitrate was the control.Genomic DNA was extracted at30h.Then Agarose gel electrophoresis was performed RESULTS: DNA degradation or cross-linkage was produced by high concentrations of lanthanide ions. CONCLUSION: Lanthanide ions affected degradation and crosslinking on genomic DNA of E.coli.
【Fund】: 安徽省教育厅自然科学基金项目(No.2005KJ197)
【CateGory Index】: Q933
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