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《Carcinogenesis,Teratogenesis & Mutagenesis》 2006-03
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Isolation and Purification of Antitumor Component in Rhizoma Menispermi and Analysis of Its Activity

SHAN Bao-en1, REN Feng-zhi2, LIANG Wen-jie1,3,ZHANG Jing1,Li Qiao-xia1, ZENG Ya-ping4(1.Research Center, the Fourth Hospital of Hebei Medical University, Shijiazhuang, 050011,China;2.North China Pharmacal Coperation China;3.Hebei Medical University Shijiazhuang 050011,China;4.Hebei Science and Technology Publishing House,Shijiazhuang,050073, China )  
BACKGROUND AIM: Isolate and purify active component in Rhizoma Menispermi extract and analyze the activity of the active component. MATERIAL AND METHODS: The suppressive effects of Rhizoma Menipermi extracts on the proliferation of tumor cells were assayed in vitro using MTT colorimetric method. Rhizoma Menipermi extract with ethanol was preliminarily purified using three steps method. PE2 was isolated and purified further by Pole Chromatography. The components were identified by Thin Layer Chromatography、ESI-MS and Ultraviolet Spectro- photometry. RESULTS: Rhizoma Menipermi extracts RMW and RME inhibited markedly the proliferation of tumor cells such as K562、BGC823 and TE13 in a dose-and time-dependent manner(P0.01). But Rhizoma Menipermi extract RMP had no inhibitory effect. The two compounts, PE1 and PE2 seperated from RME, also had very strong inhibitory effect(P0.01). Through active component analysis, PE2 was found to contain some alkloids. PE2 was purified further and then PF1 and PF2 were isolated, with molecular weights of 624 and 610, respectively. PF1 and PF2 inhibited markedly the proliferation of tumor cells such as K562、BGC823 and TE13 (P0.01). PF2 had stronger inhibitoryeffect than PF1. PF2 could induce morphologic changes of K562、BGC823 and TE13 cells. PF2 also inhibited the proliferation of tumor cells from primary culture of tumor tissues of patients (P0.01). CONCLUSION: Both RMW and RME had very strong antitumor effects in vitro.The effect of RME was stronger than RMW. PE2 was the active antitumor part of RME. PF1 and PF2 were the active antitumor components of PE2, and were probably Dauricine and Daurisoline,respectively. RMP had no inhibitory effect on tumor cells.
【Fund】: 国家自然基金 No.3037153;; 河北省自然基金 No.C2004000610
【CateGory Index】: R284
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