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《Carcinogenesis,Teratogenesis & Mutagenesis》 2006-04
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Effect on Apoptosis of BGC823 Cells Induced by Rhizoma Menipermi Extracts-PE2

SHAN Bao_en 1 ,LIANG Wen_jie 1 ,LIU Dong_qing 2 ,REN Feng_zhi 3 (1.Research Center,The Fourth Hospital of Hebei Medical University,Shijizhuang,2.The First Hospital of Handan; 3.Northchina Phamacal coperation,050011,China)  
BACKGROUNDAIM: To study the effects of tumor cell apoptosis induced by Rhizoma Menipermi Extracts_PE2and their mechanisms. MATERIAL AND METHODS: Rhizoma Menipermi extract with ethanol was preliminary purified using three steps method.PE2was isolated and purified further by Pole Chromatogram.The apoptosis of tumor cells BGC823induced by PE2was studied by fluorescent staining,flow_cytometry(FCM),electron microscopy and DNA agarose gel electrophoresis technique. RESULTS: PE2could induce apoptosis of BGC823cells in a dose_and time_dependent relationship.After treatment with PE2,the BGC823cells received some typical morphologic features and super_microstructural changes of apoptosis,including cell shrinkage chromatin concentrating on nuclear membrane or forming crescent,nuclear condensation,nuclear fragmentation and formation of apoptotic bodies.Some typical subdiploid peaks before G 0 /G 1 phase were observed.DNA agarose gel electrophoresis showed characteristic”DNA ladder”pattern.After treatment with5μg/ml PE2,the cells in G 0 /G 1 phase were blocked and increased markedly.But the cells in S phase were blocked after treatment with50μg/ml PE2.And the expression of bax was up_regulated. CONCLUSION: Rhizoma Menispermi Extract_PE2induced tumor cell apoptosis,which was an important mechanism of its anti_tumor effect.Futher purification and study of their mechanisms can produce the scientific evidence for developing and manufacturing new antitumor drugs.
【Fund】: 国家自然基金 No.3037153;; 河北省自然基金 No.C2004000610
【CateGory Index】: R735.2
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