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《Carcinogenesis,Teratogenesis & Mutagenesis》 2006-04
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Gene Transfer Mediated by Liposome to Spermatogonial Cell

YANG Xing_kun,HUANG Tian_hua ˇ , XIE Qing_dong,XIONG Xiao_fang,WU Cong_mei (Research Center of Reproductive Medicine,Shantou University Medical College,Shantou515041,Guangdong,China)  
BACKGROUNDAIM: To investigate the efficacy of transfection of spermatogonial stem cells with pDsRed2_N1reporter by liposomes. MATERIAL AND METHODS: Red_fluorescent protein(DsRed2)genes were mediated by DOSPER liposomes to spermatogonial stem cells of donor mice in vitro and in vivo.The DsRed2gene_transfected cells,after isolation and purification,were transplanted into the seminiferous tubules of anesthetized recipient mice depleted of germ cells by busulfan treatment. RESULTS: The expression rate of DsRed2gene was20%of in vitro transfected cells and higher than8%of in vivo transfected cells(P0.01).Spermatogonial stem cells with DsRed2expression were detected on frozen section of testis from the recipient mouse6wafter transplantation. CONCLUSION: This may provide a simple and effective approach to study spermatogenesis mechanism and to produce transgenic animals using combination of DOSPER_mediated gene transfer in vitro with spermatogonial cell transplantation.
【CateGory Index】: Q813
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