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《Carcinogenesis,Teratogenesis & Mutagenesis》 2007-06
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Inhibitory Effects of Phellinus Linteus Intracellular Polysaccharide on the Proliferation of Leukemic Cells K562

SHI Xin-qiang,SHEN Ye-shou,WEI Zi,MA Jin-bao(Institute of Biochmistry and Microbiology,School of Life Science,Anhui Universitya;The Key Lab of Chinese Traditional Medicine Research and Development of Anhui Province,Hefei 230039,China)  
BACKGROUND AIM:To examine the inhibitory effects of Phellinus linteus intracellular polysaccharide(PLIP) on proliferation of leukemic cell line K562,and to explore its preliminary mechanisms.MATERIALS AND METHODS:The suppression of cellular growth was detected by MTT assay and trypan blue rejection.Apoptosis was studied by using Hoechst-PI fluorescence staining,DNA agarose gel electrophoresis,and flow cytometry.RESULTS:The PLIP could significantly inhibit the proliferation of K562 cells(P0.05),and the highest inhibition rate could reach 52.55% at the dose of 400 μg/ml,IC50 was 262.36 μg/ml.The apoptosis rates for 100 μg/ml,200 μg/ml,400 μg/ml were 5.72%,13.57%,19.39%,respectively,and morphologic and physiologic changes of apoptosis in K562 cells were induced.CONCLUSION:PLIP could significantly inhibit proliferation of K562 cells,and the mechanism responsible for the effect might involve the induction of apoptosis and the effects on cell cycle.
【CateGory Index】: R733.7
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