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《Carcinogenesis,Teratogenesis & Mutagenesis》 2008-06
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Effect of Baohuoside-Ⅰon Apoptosis of Human Esophageal Carcinoma Cell Eca-109

LIU Xiao-xia1,SHAN Bao-en2,,CHEN Yu-min1,REN Feng-zhi3,SHANG Xiao-hui1(1.Medical College of Hebei University of Engineering,Handan 056029 Hebei,China;2.Research Center,Fourth Hospital of Hebei Medical University,Shijiazhuang 050011 Hebei,China;3.New Drug Research and Development Co.Ltd.of North China Pharmaceutical Corporation,Shijiazhuang 050015,Hebei,China)  
BACKGROUND AND AIM:Effect of baohuoside-from Cortex Periplocae on apoptosis of human esophageal carcinoma cell Eca-109 and its mechanism were studied.MATERIALS AND METHODS:After treatment with baohuoside-at different concentrations(12.5,25,50 μg/ml)for 24 h,48 h,72 h,the inhibitory effect on proliferation of Eca-109 cells was analyzed by MTT method.After treatment with baohuoside-under different concentrations(12.5、25、50 μg/ml) for 48 h,cell apoptotic ratio and expression of Eca-109 cells Survivin protein of were measured with flow cytometry(FCM);the ultrastructure was examined by transmission electron microscope;the expression of Survivin mRNA was detected by RT-PCR.RESULTS:Baohuoside-significantly inhibited proliferation of Eca-109 cells,and in concentration-dependent manner(P all0.05),the IC50 was 24.8 μg/ml.After treatment with different concentrations of Baohuoside-Ⅰfor 48 h,cell apoptosis of Eca-109 cells was induced significantly(the apoptotic ratio is 55.26% treated with 50 μg/ml of baohuoside-) and showing characteristic ultrastructural changes of apoptosis.Expression levels of Survivin mRNA and protein were decreased significantly(P0.01).CONCLUSION:Baohuoside-of Cortex Periplocae could inhibit the proliferation and induce apoptosis of Eca-109 cell.This effect was associated with down-regulation of Survivin mRNA expression.
【Fund】: 国家自然科学基金资助项目(30772752)
【CateGory Index】: R735.1
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