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《Carcinogenesis,Teratogenesis & Mutagenesis》 2009-02
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Clinicopathological Significance of p53 Gene Expression and Telomerase Activity in Circulating Tumor Cells of Patients with Colorectal Carcinoma

ZHOU Jian-nong1,MA Guo-jian2,HE Xiao-song2,SHEN Zong-li2,XUE Kai-xian2(1.Department of General Surgery,Jiangsu Cancer Hospital,Nanjing 210009;2.Laboratory of Genetics,Jiangsu Institute for Cancer Research,Nanjing 210009,Jiangsu,China)  
BACKGROUND AND AIM:To study the clinicopathological significance of p53 gene expression and telomerase activity in circulating tumor cells of patients with colorectal carcinoma.MATERIALS AND METHODS:Mononuclear cells containing circulating tumor cells were isolated by Ficoll-Hypaque gradient centrifugation in peripheral blood from 113 preoperative patients with colorecatal carcinomas,13 patients with benign colorectal disease,and 28 healthy volunteers.FCM and TRAP-ELASA were used to evaluate p53 gene expression and telomerase activity,respectively.Experimental data were analyzed by SPSS 11.0 software and χ2 test.RESULTS:Among 105 informative patients with colorectal carcinoma,positive rate of p53 expression and telomerse activity were 74.3%(78/105)and 61.0%(64/105),respectively.Aberrant expression levels of both p53 gene and telomerase in patients with colorectal cancer were significantly correlated with lymph node metastasis(P0.05)and their simultaneous measurement could raise the prediction accuracy of metastasis.Expression of p53 gene was also significantly correlated with differentiation degree of colorectal carcinoma(P=0.003).CONCLUSION:Aberrant p53 gene expression and telomerase activity in circulating tumor cells might be malignant biomarkers.Both were closely related and could predict nodal involvement.
【Fund】: 江苏省科技厅基金资助项目(BS99056)
【CateGory Index】: R735.34
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