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《Carcinogenesis,Teratogenesis & Mutagenesis》 2010-03
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Effects of triterpenes compound of cortex periplocae on regulatory T cells function in N-nitrosomethylbenzylamine-induced esophageal tumorigenesis in F344 rats

WANG Li-fang1,SHAN Bao-en2,SHAN Tie-qiang2(1.Laboratory Department of the Second Hospital of Hebei Medical University,Shijiazhuang 050000;2.Research Center,the Fourth Hospital of Hebei Medical University,Shijiazhuang 050011,Hebei,China)  
OBJECTIVE:To investigate the effects of triterpenes compound of cortex periplocae(TCCP)on CD4+ CD25+ regulatory T cells in peripherial blood in N-nitrosomethylbenzylamine(NMBA)-induced esophageal tumorigenesis in F344 rats.METHODS:40 male F344 rats(5-6 weeks of age)were randomly divided into four groups:rats were treated with 0.5 mg/kg NMBA only as model group and rats received NMBA 0.5 mg/kg s.c.plus TCCP 10 mg/kg i.m.as TCCP treatment groups and those treated with soya oil 1 ml/kg intramuscularily(i.m.)acted as soya oil control and normal control groups.10 rats in each group were treated thrice per week for 5 weeks.Then the numbers of CD4+ and CD4+CD25+ regulatory T cells in peripheral blood were counted by flow cytometry at weeks 9 and 15.The levels of IL-2,IL-10 and TGF-β1 in peripheral blood were detected with ELISA.RESULTS:After 0.5 mg/kg NMBA treatment for 9 weeks,CD4+ T cells in peripheral blood of NMBA group rats were decreased significantly whilst CD4+CD25+ regulatory T cells were significantly increased compared with the control group(P0.05).At weeks 9 and 15,TCCP treatment decreased the proportions of CD4+CD25+ regulatory T cells,while the proportions of CD4+ cells were significantly increased(P0.05),compared with NMBA group.At week 9 after NMBA treatment,the protein levels of TGF-β1 and IL-10 in peripheral blood were significantly higher than those in normal control group and soya controlgroup.However the protein levels of IL-2 were significantly decreased,as compared with normal control.TCCP could decrease the expression of IL-10 and TGF-β1,but increase the expression of IL-2.CONCLUSION:In rats the expression of CD4+CD25+ regulatory T cells in peripheral blood were enhanced after treatment with NMBA,while the triterpenes compound of Cortex Periplocae effectively inhibited the expression,thus it could improve the immune dysfunction in experimental esophageal tumorigenesis in F344 rats.
【Fund】: 国家自然科学基金(30772752);; 河北省自然科学基金(C2008000952)
【CateGory Index】: R735.1
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