Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Carcinogenesis,Teratogenesis & Mutagenesis》 2014-03
Add to Favorite Get Latest Update

The effects and mechanisms of oxymatrine on anti-influenza A virus activity

CHEN Xiao-xuan;DAI Jian-ping;WAN Qian-ying;ZHU Dan-xia;WANG Ge-fei;LI Kang-sheng;Department of Microbiology and Immunology, Shantou University Medical College;  
OBJECTIVE: To explore the effects and mechanisms of oxymatrine on anti-influenza A virus(IAV) activity. METHODS: MDCK and A549 cells were cultured and divided into 4 groups: blank control(DMSO, 0.5%), virus-treated group,positive group(ribavirin,200 μmol/L) and oxymatrine-treated group(12.5,25,50 and 100 μmol/L),with 6 repeats in each group. The anti-IAV activity of oxymatrine was determined by plaque inhibition assay. The influence of oxymatrine on the IAV-induced transcription of TLR4,MyD88 and TRAF6,the activation of TLR4-Myd88-TRAF6-NF-κB signal pathway,and the release of inflammatory cytokins were determined by luciferase reporter plasmid,Western blot and ELISA assays,respectively. RESULTS: Plaque inhibition assay showed that oxymatrine, at a range of 12.5-100 μmol/L,could significantly inhibit the replication of IAV in vitro,and its EC50 was 19.95 μmol/L. Luciferase reporter assay showed that oxymatrine,at the concentration of 12.5 μmol/L,could significantly inhibit the transcription of TLR4,MyD88 and TRAF6 induced by IAV infection(compared with virus-treated group,P 0.05). Western blot assay showed that oxymatrine(12.5 μmol/L) could significantly inhibit the IAV-induced activation of TLR4-Myd88-TRAF6-NF-κB pathway(compared with virus-treated group,P0.05). ELISA assay showed that oxymatrine(12.5 μmol/L) could significantly inhibit the IAV-induced release of inflammatory cytokins(compared with virus-treated group,P 0.05). CONCLUSION: Oxymatrine possessed anti-IAV activity,and its mechanism may be related to its ability to inhibit the IAV-induced activation of TLR4-MyD88-TRAF6-NF-κB pathway.
【Fund】: 国家自然科学基金项目(81374040)
【CateGory Index】: R285
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved