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《CARCINOGENESIS, TERATOGENESIS AND MUTAGENESIS》 1997-04
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STUDIES ON THE METHODOLOGY FOR QUANTITATIVEDETERMINATION OF TELOMERASE ACTIVITY

Chen Tiehe,Yin Muquan,Bi Jie,Chen Yaofu,Zhu Bingchai Department of Health Toxicology,Second Military Medical University,Shanghai 200433  
In this paper,an assay of quantitative determination of telomerase activity has been studied.And some fators such as reacting system and conditions,methods of isolation and fixation,and so on ,which may influence the reproduction were also investigated.Results showed that the inter-batch and extra-batch variations were 15.6% and 16.4%, respectively.It revealed well linear relationship between telomerase activity and the value within 700 ̄7000/min determined by radioactive count.The telomerase activity of some specimens of cancer results from the procedures of this assay were accordant with literature.
【CateGory Index】: Q55
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【References】
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1 Chen Tiehe; Yin Muquan; Zhu Bingchai; Cai Ping(1 The Institute of Naval Medical Research, Shanghai 200433)(2 The Second MiIitary Medical University, Shanghai 200433);Telomerase as a Fetal Tumor Marker[J];BASIC MEDICAL SCIENCES AND CLINICS;1999-05
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