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《CARCINOGENESIS, TERATOGENESIS AND MUTAGENESIS》 1998-06
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EFFECTS OF VARIOUS ANTIOXIDANTS ON UV INDUCED THE FORMATION OF HUMAN SKIN'S CYCLOBUTYL PRIMIDINE DIMERS

E Xiaofei 1, Zhu Xudong 2, Li Jing 1, Liu Yang 1, Sun Xinyan 3, Wang Bingxian 1 1Department of Environmental Health, School of Public Health, China Medical University\ 2Department of Center Laboratory, The first Clinical College, 3Department of Anaes  
To define further the role of reactive oxygen species (ROS) in ultraviolet radiation (UV) induced the formation of Human skin's CPD, experiments were performed using alkaline gel electrophoresis technique. Purified DNA was incubated with antioxidants. After DNA was exposed to UV, T4 Endonuclease V was added, electrophoresis was taken, CPD was measured by Gel Imaging and Image Analysis. It was found that antioxidants exhibited some inhibitory effects on the formation of CPD, β carotene and superoxide dismutase (SOD) are more obvious than GSH and CAT, but none of which were able to block the formation completely. The results suggest that the formation of CPD is only partly dependent on ROS.
【Fund】: 国家自然科学基金
【CateGory Index】: R-33,
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