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Study on Transformation of Wheat and Maize Via Gene-gun Bombardment

Liu Jinyuan, Shi Xiangyu, Chen Xiao, Zeng Zhaohai,Zhou Zhuhua & Chen Jianai(Institute of Atomic Energy Application in Agriculture,Shandong Acodemy of Agricultural Sciences,Jinan 250100)  
Calli of wheat and maize were subjected to JQ-700(made in China)gene-gun bombardment,using plasmid pHB101 containing Bt-gene as donor.32 regenerated wheat seedlings and 14maize resistant plants were obtained by Hygromyain resistance selecting .15 regenerated wheat resistant seedlings were confirmed to show positive signal by dot hybridization blotting test,using pHB101 plasmid labeled by a-32p-dCTP as probe.And the progeny of one plant appeared good resistance to aphid and rust in the field. One stable line resistant to corn borer had been selected in the progeny of maize regenerated plants, further molecular test and feeding-insect test are going on.
【CateGory Index】: S512.103
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