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《Shandong Agricultural Sciences》 2002-01
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A preliminary study on genetic identification and purity assessments of garlic by RAPD

XU Pei-wen 1,QU Shi-song 1,HUANG Bao-yong 1,SHI Yan-jing 2 LIU Heng-ying 1,ZHANG Jie 1,SUN Jin-bin,SONG Hong-ping 1 (1 Institute of Vegetable Research, Shandong Academy of Agricultural Sciences,Jinan 250100,China; 2 Department of Horticulture,  
random primers have been used to screen for useful primers in study on genetic identification and purity assessments by RAPD, from which 12 useful primers were selected and used in RAPD assay with 80 garlic cultivars and lines mainly collected from 17 provinces and regions of China and abroad The optimum reaction system and program of temperature adjustment for PCR has been worked out and RAPD banding patterns of the garlic germplasm showed good polymorphism and specificity Differences in the garlic based on the banding patterns presented the same tendency with that by agronomic characters Confusions by one garlic cultivar with different names or one name given to several garlic cultivars have been made clear Purities of several garlic cultivars were tested It is depicted that the RAPD technique will be one of the useful tools in genetic identification and purity assessments of garlic
【Key Words】： Garlic germplasm RAPD genetic purity
【Fund】： 山东省农科院重点科技攻关项目;; 中国农业部、瑞士科技发展部、亚洲蔬菜研究与发展区域中心合作项目资助
【CateGory Index】： S633.403.8
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