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《Shandong Agricultural Sciences》 2003-02
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Purity detection of Lumianyan 15 hybrid with SSR markers

LIU Qin-hong,WANG FU-rong,ZHANG Jun,YANG Jing,LI Ru-zhong,WANG Zong-wen(Shandong Cotton Research Center, Jinan 250100, China)  
The main problem of hybridized cottonseeds was intermixing with the female parent resulted from the no-thorough castration The traditional way of purity detection was performed in the next cotton growth season and could not reliably detect the purity of the hybridized cottonseeds before the growth season In this study, more than ten SSR marker loci which could be used to distinguish Lumianyan 15 F 1 from its parents were discovered through screening 217 pairs of SSR primers from allotetraploid cotton A reliable, stable, rapid and useful technique was provided to detect purity of hybridized cottonseeds of Lumianyan 15
【Fund】: 山东省科技厅农业良种产业化资助课题 ;; 山东省农科院重点学科“棉作学”资助课题
【CateGory Index】: S562
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