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《Shandong Agricultural Sciences》 2003-04
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Induction and identification of haploids of advanced hybridization of transgenic Bt upland cotton

ZHANG Jun1,Wang Fu-rong1,LIU Qin-hong1,BAI Jin2,LIU Ren-zhong1(1Shandong Cotton Research Center, Jinan 250100,China;2Shandong Agricultural University,Taian 271018,China)  
The progress of crop breeding can be promoted via doubled haploid technique that can rapidly polymerize and purify several advanced genes The virescently marked semigamy line(vSg) in island cotton (Gossypium barbadense L) has potential to produce high frequency of haploids,and can be used as female parent to induce target haploids In the present study, the haploids of advanced hybridization of Bt transgenic upland cotton (Gossypium hirsutum L) were successfully induced by vSg It is difficult to identify the haploid individuals in the early developmental stage of the offspring population of the cross between vSg and the target hybridization crosses An effective method was proposed to detect the vSg induced target haploids through Bt gene screening and molecular marker identification
【Fund】: 山东省科技厅农业良种产业化资助课题 ;山东省农科院重点学科“棉作学”资助课题。
【CateGory Index】: S562.035
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