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《Shandong Agricultural Sciences》 2004-03
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Studies on transformation of Bt. gene into maize by micro-particle bombardments

SUN Hong-wei~1, ZHU Chang-xiang~2,YANG Chong-liang~1, SHANG You-fen~1, ZHAO Jiu-hua~1, LU Xing-bo~1, WANG Sheng-ji~1 (1.Plant Protection Institute, Shandong Academy of Agricultural Science, Jinan 250100,China; 2.Plant Protection college, Shandong Agricultural University, Taian 271018,China)  
By micro-particle bombardments, Plasmid pBW3 which is component of Bt.and Bar gene was transferred into maize immature embryos of maize self-bred lines A188, resistant maize plants were obtained.Dot-bloting and Southern-bloting of transgenic maize leaves showed:Bt.gene had integrated into resistant maize plants, heterogeneous gene had four copies in maize gene group.
【CateGory Index】: S513
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