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《Shandong Agricultural Sciences》 2004-06
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The regeneration technology system of axillary bud from phalaenopsis wisonii flower stems with tissue culture

ZhANG Yuan-guo~1,DIAO Jia-lian~1,lIU Yu-e~2,LI Fang~1,BAO Yan-cun~1,DIAO Xi-qiang~1 (1.Weifang Academy of Agricultural Sciences,Weifang,261041;2.Weifang Profession Academy,Weifang,261041)  
The vegetative buds were induced from flower stems axillary buds.The Protocorm-Like-Body(PLB) were induced from the shoot tips. The regeneration technology system of phalaenopsis was established. The flower stems axillary bud inducing、shoot tips inducing PLB, regeneration, rooting, transition medium were selected.The transition cultivate technology was studied. The shoot rate, PLB induction rate, rooting rate and transition cultivate survival rate were 90%、100%、100% and 90%respectively.
【Fund】: 山东省科技攻关课题"绿化苗木转基因技术研究"和潍坊市科技攻关项目"蝴蝶兰组培苗转绿色荧光基因技术研究"的部分内容。
【CateGory Index】: S682.31
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