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《Shandong Agricultural Sciences》 2005-03
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Marking of high molecular glutenin 1Bx14 subunit using specific PCR marker in Shandong winter wheat cultivars

XU Miao1,LI Ting-bo2,WANG Xian-ze1(1.Life Science College, Shandong Agricultural University, Taian 271018;2. Dongchangfu District Agricultural Bureau, Liaocheng 252000)  
A pair of specific primers designed based on the known wheat 1Bx14 gene sequence were applied to amplify 7 wheat cultivars with known HMW-GS in Glu-Bx14 locus. The results indicated that 419bp specific bands were appeared in all wheat cultivars amplified with 1Bx14, which was in coincidence with the results of SDS-PAGE. 20 wheat cultivars in Shandong province were screened applying the specific marker, and 7 cultivars were found out with 1Bx14 gene. Compared with SDS-PAGE, PCR marker was more rapid, handy and exact.
【Fund】: 山东省三零工程项目部分研究内容
【CateGory Index】: S512.1
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