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《Shandong Agricultural Sciences》 2006-06
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Study on the High Efficient Tissue Culture System of Pumpkin

ZHANG Wei-hua~1,SUN Xiao-lei~1,WANG Zhi-feng~1,CAO Qi-wei~1,LIU Wen-bao~2(1.Research Institute of Vegetable,Shandong Academy of Agricultural Sciences,Jinan 250100,China;2.Development Corporation of Agricultural Science and Technology,Jinan 250100,China)  
Effects of seedling age,the hormone combination,different explants,the uime of shoot induction and different genotypes on pumpkin tissue culture efficiency were studied.The results showed that: when the pumpkin cotyledons from 3~5 days seedling were induced in the medium MS+6-BA 1 mg/L+Ades 20 mg/L for about 10 days,and then transferred into shoot elongation medium,MS+GA_3 0.5 mg/L,more plantlets could regenerate rapidly from different genotype explants.The time from cotyledon subculturing to plantlet transplanting needed only 4~5 weeks.
【Fund】: 山东省“三○”工程资助项目
【CateGory Index】: S642.1
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