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《Shandong Agricultural Sciences》 2007-03
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Pilot Study on the Transformation Technology of Muskmelon Inbred Line by Pollen-tube Pathway

HOU Li-xia1,HE Qi-wei1*,BU Li-xia2,WANG Chong-qi1,DONG Yu-mei1,JIAO Zi-gao1(1.Vegetable Institute,Shandong Academy of Agricultural Sciences,Jinan 250100,China;2.Development Bureau of Agriculture economics in District Huaiyin,Jinan 250117,China)  
The experiments using pollen-tube pathway to transform three muskmelon inbred lines,and selecting suitable concentration of Kan to detect transformation plants early and quickly were carried out.The results showed that the transformation efficiency of microinjection was higher than that of stigma-dropping.Cutting stigma and dropping repeatly was profit for transformation of extraneous gene.The transformation efficiency of three muskmelon inbred lines was 0~1.4% and there were differences between them.The average transformation efficiencies of TS1,TS2 and TS3 were 0.30%,0.225% and 0.25%.It was feasible to detect transgenic seeds early by critical concentration of Kan,and the accuracy efficiency of detecting cotyledons was higher.PCR results of seedlings resistant to Kan showed that transforming muskmelon by pollen-tube pathway was successful.
【CateGory Index】: S652
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