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《Shandong Agricultural Sciences》 2007-05
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Optimization of A-PAGE for Isolating Foxtail Millet(Setaria Italica L. Beauv.) Salt-soluble Proteins

ZHANG Hua-wen1,YANG Yan-bing1,QIN Ling1,GUAN Yan-an1,DUAN Nai-bin1,NI Da-peng2,YU Dong-an(1.Crop Research Institute,Shandong Academy of Agricultural Sciences,Jinan 250100,China;2.Institute for Application of Atomic Energy,Shandong Academy of Agricultural Sciences,Jinan 250100,China)  
The experiment was conducted to optimize the conditions of A-PAGE for isolating salt soluble proteins of foxtail millet.10 foxtail millet varieties were used for A-PAGE.A number of A-PAGE electropherograms were obtained at different levels of dosage of catalyst,gel concentrations,cross-linkages,injecting volumes,pH of gel buffer,and the use of inspissate gel.The optimal conditions for A-PAGE were raised after deliberate investigation.Detailed criteria were as following: dosage of 3% H2O2 1 μl/ml gel,gel concentration 13%,cross-linkages 4%,pH of gel buffer 3.7,and the injecting volumes between 20 and 30 μl.Furthermore,the use of inspissate gel enhanced the resolution of gel electropherograms.
【Fund】: 农业结构调整重大技术研究专项“高效优质谷子新品种选育及配套栽培技术研究”(05-05-01B);; 山东省农业科学院青年基金项目“利用DNA指纹和蛋白质图谱进行谷子品种真实性鉴定的研究”(2005YQ051)资助
【CateGory Index】: S515
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