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《Shandong Agricultural Sciences》 2008-01
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A Simple Method for Separation and Purification of High and Low Molecular Weight Glutenin Subunits in Wheat

LI Qiao-yun1,2,LI Shi-ping3,ZHANG En-ying1,PEI Yu-he1,SONG Xi-yun1(1.College of Life Science,Qingdao Agricultural University,Qingdao 266109,China;2.Institute of Vegetable Crops,Shandong Academy of Agricultural Sciences,Jinan 250100,China;3.Dezhou Academy,Dezhou 253023,China)  
Glutenin was extracted from common wheat.Two types of polydextran gel Sephadex G-50 and Sephadex G-100 were used for study of separation and purification of High and Low Molecular Weight Glutenin Subunits by chromatography.The effects were checked by the method of SDS-PAGE.Protein concentration of each fraction was measured by the method of UV spectrophotometer,and the reclaim ratio of protein was calculated.The results showed that protein reclaim ratio of Sephadex G-50 was higher,and it could be used for purification of LMW-GS,while not for HMW-GS.Sephadex G-100 could separate High Molecular Weight Glutenin Subunits from Low Molecular Weight Glutenin Subunits effectively,but compared to Sephadex G-50,its reclaim ratio of protein was lower.
【CateGory Index】: S512.1
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【Co-citations】
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