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《Shandong Agricultural Sciences》 2008-01
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Studies of Transgenic Wheat D_1 by Introducing Spirulina Total DNA through Pollen Tube Pathway

HU Wen-ming1,2,XU Cui-lian1,WANG Yue-guang2(1.College of Plant Science and Technology,Tarim University,Alar 843300,China;2.Qingdao Agricultural University,Qingdao 266109,China)  
The total DNA of spirulina was introduced into wheat varieties 9411,955159,96266 and Lumai21 through pollen tube pathway.The D1 genration of transgenic wheat was studied and the results were as follows.Compared to the control,the germination percentage of the D1 seeds decreased by 6.25%~20.0%.The average plant height of transgenic 9411 and Lumai21decreased 5 and 4.6 centimeter respectively.The variation of awnless ears were found in transgenic D1 of 955159 and Lumai21.The variation rates were all up to 5% of chromosome number,lagging and bridge chromosome in root tip cells.The content of crude protein decreased at different degree.The high weight subunit of glutenin varied in transgenic D1 of 9411 and Lumai21.
【CateGory Index】: S512.1
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