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《Shandong Agricultural Sciences》 2008-01
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An Improved CTAB Method for Extracting Total RNA from Walnut

LIU Xiao-ju1,HONG Hai-bo2,LI Min1,LIU Yuan1,YANG Ke-qiang 1(1.College of Forestry,Shandong Agricultural University,Taian 271018,China;2.Haidong Agricultural Extension Center in Qinghai,Haidong 810600,China)  
Extracting high-quality RNA from plant tissue is the prerequisite and key to carrying out researches in molecular biology.Because different tissues of different plant species differ in component composition,RNA extraction have different difficulties.According to the prior researches,a highly efficient RNA extraction method of CTAB was improved in light of the complicated inclusion in walnut leaves.The results showed clear bands and indicated that the RNA was of high purity and good quality and was suitable for RT-PCR and the construction of cDNA library.It is believed that the CTAB method is especially useful for the RNA extraction of plant materials which have plentiful polysaccharide,hydroxybenzene,pigment and other secondary metabolites.Furthermore,this method is simple,economic and of stable performance and good repeatability.
【Fund】: 山东省人事厅项目(200603039)
【CateGory Index】: S664.1
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