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《Shandong Agricultural Sciences》 2008-03
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Sequencing and Analysis of US2 Gene of Duck Enteritis Virus

LI Zi-jian1,LI Yun-long1,CHEN Su1,AI Wu2,SONG Min-xun2,LI Yu-feng2 (1.College of Life Science,Shandong Normal University,Jinan 250014,China;2.Institute of Poultry Science,Shandong Academy of Agricultural Sciences,Jinan 250023,China)  
The genomic DNA of DEV SD-01 strain was extracted and a pair of primers was designed according to the published nucleotide sequence in GenBank.The US2 gene was amplified by polymerase chain reaction(PCR) with extracted total DNA from DEV SD-01 strain as template and cloned into vector pGM-T.The positive recombinant plasmid was named pGM-US2 and sequenced.The results showed that the homology of the nucleotides and amino acid of US2 gene from DEV SD-01 strain with that of DEV vaccine strain,C-CKE strain,HSV-1,MDV and PRV were 100%~25.8% and 100%~28% respectively.These data suggested that US2 gene was completely conserved among different DEV strains,whereas showing low level homology with other herpesviruses.
【Fund】: 山东省自然科学基金项目(编号:Y2007D70)
【CateGory Index】: S852.65
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