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《Shandong Agricultural Sciences》 2008-05
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Optimization of SRAP-PCR Reaction System on Peanut

CHEN Jing,HU Xiao-hui,MIAO Hua-rong,ZHANG Jian-cheng,YU Shan-lin(Shandong Peanut Research Institute,Qingdao 266100,China)  
The concentrations of MgCl2,dNTP,Taq and primer in the SRAP-PCR system were optimized for genomic DNA of peanut.The results showed that the optimized concentrations were MgCl2 at 2.500~3.250 mmol/L,dNTP at 0.1875~0.2500 mmol/L,primer at 130 ng and Taq at 2 U,with a total volume of 20 μl.
【Fund】: 山东省良种工程(高产优质出口专用花生新品种培育)、国家科技支撑计划课题(2006BAD01A04)
【CateGory Index】: S565.2
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Chinese Journal Full-text Database 1 Hits
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Chinese Journal Full-text Database 7 Hits
1 TAN Zhen - xin, LI Ru - yu, LI Qun, YAN Ting - jin,ZHANG Wen - lan, SONG Guo - an, HOU Shu - zhen ( Crop Research Institute , Shandong Academy of Agricultural Sciences , Jinan 250100, China);Study on purity testing methods for maize hybrids using SSR markers[J];Shandong Agricultural Sciences;2004-04
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