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《Shandong Agricultural Sciences》 2008-06
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Two Fast Methods for Total RNA Extraction from Root Tubers of Sweet Potato

MA Li(Department of Biology,Zaozhuang College,Zaozhuang 277160,China)  
The root tubers of sweet potato are rich of secondary metabolites,which hinder the isolation of RNA.Guanidine Thiocyanate-one step method and CTAB method were used to isolate the total RNA from tubers of sweet potato.The results showed high purity and integrated RNA was obtained by improved methods.The high quality total RNA was obtained by CTAB method with no degradation or contaminant of complicated secondary metabolites,such as polysaccharides,phenolic compounds and fibrin.
【CateGory Index】: S531
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