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《Shandong Agricultural Sciences》 2008-08
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Screening for Strain with High Laccase Activity by Protoplast Electrofusion of Inactivated Parental Strains

REN Xuan,JIA Le*,SONG Zhen,DENG Peng(College of Life Science,Shandong Agricultural University,Taian 271018,China)  
With Pleurous eryngii and Pleurotus ostreatus as parents,the strain with high laccase activity and genetic stability was screened by protoplast elecrofusion of the inactivated parental strains in this paper.With UV method,Pleurous eryngii was handled at 30 W,irradiation distance of 30 cm for 20 minutes,and the lethality rate was 100%;with the method of heat inactivation,Pleurotus ostreatus was handled at 65℃ for 30 minutes,and the lethality rate was also 100%.The strains of Pleurous eryngii and Pleurotus ostreatus were mixed by 1∶1 and electrofused in the alternating electric field with frequency of 1 MHz,intensity of 250V/cm and fusion pulse intensity of 5.5 kV/cm.The pairing rate of protoplasts arrived at 70%and the fusion rate was 3.2× 10-3.One strain with characters of both parents was picked out from 147 fusion strains and the enzyme activity of laccase and TTC-dehydrogenase increased by 20.6% and 25.3% respectively than its parents while their heredity stability arrived at 92.0% and 92.7%.The fusant was identified based on colony character,morphological state,growth rate and antagonistic effect,then the screened strain was confirmed with the characters of parents.
【Fund】: 山东省自然科学基金资助项目(Y2006D08)
【CateGory Index】: S646.13
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