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《Shandong Agricultural Sciences》 2009-03
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Studies of Optimum RAPD-PCR Conditions for Pleurotus ostreatus

REN Hai-xia,GONG Zhi-yuan,LI Jin,REN Peng-fei,YAO Qiang,QU Ling (Institute of Soil and Fertilizer of Shandong Academy of Agricultural Sciences,Jinan 250100,China)  
With the variety Heifeng 268 as material,the reaction system of RAPD-PCR for Pleurotus ostreatus was studied.The results showed that the optimum conditions were as follows: 20 μl PCR reaction volume,1.4 mmol/L Mg2+,50 ng template DNA,0.6～0.8 μmol/L primer,250 μmol/L dNTPs,1.2 U Taqase and 2.0 μl 10×Buffer.
【Key Words】： Pleurotus ostreatus RAPD-PCR Factors
【Fund】： 山东省农业科学院青年基金项目(2006YQN028);; 农业部行业科技专项项目“华北地区食用菌菌种质量评价与菌种信息系统研究与建立”(nyhyzx07-008);; 山东省农业良种工程项目“山东名产和优势食用菌新品种选育”(2007LZ06)
【CateGory Index】： S646.14
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