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《Shandong Agricultural Sciences》 2009-04
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Microbial Properties and Application of Superior Bacteria in Deep-litter Ecosystem for Piggery

ZHANG Qing-ning1,HU Ming2,ZHU Rong-sheng2,REN Xiang-quan3,WU Ying2,WANG Huai-zhong2,LIU Yu-qing2,WANG Shu-bai1(1.Qingdao Agricultural University,Qingdao 266109,China;2.Institute of Animal Science and Veterinary Medicine,Shandong Academy of Agricultural Sciences,Jinan 250100,China;3.Livestock Technology Company of Dilisi Group,Zhucheng 262216,China)  
The dominant aerobic bacteria in the active deep-litter ecosystem for pigs were studied and 14 strains were isolated.All of them were identified to be Bacillus sp.and belonged to different species by morphology analysis,16S sDNA sequence analysis and homology analysis.The isolated strains were resistant to heat and eugenic on pig manure litter and some were good for the deodorization by decomposing manure with related enzymes.Furthermore,some pathogenetic bacteria were inhibited by the filtered extracellular cultures of the isolated stains.The strains were susceptible to most antibiotic and,applying to Deep-litter Ecosystem,deceased the antibiotic resistance of intestinal flora as indicated by Escherichia coli.The research indicated that Bacillus sp.play an important role in the deep-litter systems to achieve the goals: safty,saving,enviroment protection and ovecome pathogens resistance to antibiotics.
【Fund】: 抗生素残留引起细菌耐药性安全控制技术的研究与示范(2006BAK02A03-6);; 山东省安全优质猪肉生产关键技术集成熟化及可追溯产业链示范(20071HZZX11103);; 山东省农业科学院青年基金(2007YQN019)
【CateGory Index】: S828
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