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《Shandong Agricultural Sciences》 2009-05
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Elementary Exploration on Method for Separating Wheat HMW-GS by FPLC

LI Yu-bing,PEI Yu-he,WANG Hai-juan,GUO Xin-mei,ZHANG En-ying,SONG Xi-yun(College of Life Science,Qingdao Agricultural University,Qingdao 266109,China)  
As major components of wheat storage proteins,the compositions and content of high molecular weight glutenin subunit(HMW-GS) play an important role in wheat baking and processing quality.HMW-GS was separated and analyzed by gel filter with liquid chromatography methods from the common wheat Xiaoyan22.Different concentration,elution and flow rate were studied in this experiment.It was found that the concentration diluted eight times from original concentration was the best.Urea was the best elution and 0.3 ml/min was the best flow rate.
【Fund】: 国家“十一五”科技支撑计划“粮食丰产科技工程”项目(2006BAD02A09)
【CateGory Index】: S512.1
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