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《Shandong Agricultural Sciences》 2009-05
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Elementary Exploration on Method for Separating Wheat HMW-GS by FPLC

LI Yu-bing,PEI Yu-he,WANG Hai-juan,GUO Xin-mei,ZHANG En-ying,SONG Xi-yun(College of Life Science,Qingdao Agricultural University,Qingdao 266109,China)  
As major components of wheat storage proteins,the compositions and content of high molecular weight glutenin subunit(HMW-GS) play an important role in wheat baking and processing quality.HMW-GS was separated and analyzed by gel filter with liquid chromatography methods from the common wheat Xiaoyan22.Different concentration,elution and flow rate were studied in this experiment.It was found that the concentration diluted eight times from original concentration was the best.Urea was the best elution and 0.3 ml/min was the best flow rate.
【Fund】: 国家“十一五”科技支撑计划“粮食丰产科技工程”项目(2006BAD02A09)
【CateGory Index】: S512.1
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【References】
Chinese Journal Full-text Database 1 Hits
1 Li Xiaobo Kang Ligong Xu Xiangyang Li Jingfu (College of Horticulture, Northeast Agricultural University, Harbin150030, Heilongjiang Province, China);Isolation,purification and bioassay of toxin proteins secreted by different physiological races of tomato leaf mould pathogen, Cladosporium fulvum[J];Acta Phytophylacica Sinica;2010-02
【Citations】
Chinese Journal Full-text Database 5 Hits
1 LI Qiao-yun1,2,LI Shi-ping3,ZHANG En-ying1,PEI Yu-he1,SONG Xi-yun1(1.College of Life Science,Qingdao Agricultural University,Qingdao 266109,China;2.Institute of Vegetable Crops,Shandong Academy of Agricultural Sciences,Jinan 250100,China;3.Dezhou Academy,Dezhou 253023,China);A Simple Method for Separation and Purification of High and Low Molecular Weight Glutenin Subunits in Wheat[J];Shandong Agricultural Sciences;2008-01
2 ZHANG Yong 1, HE Zhong hu 1,2 , WANG Mei fang 3, ZHOU Gui ying 1, WANG De sen , ZHANG Yan 1 (1. Institute of Crop Breeding and Cultivation, Chinese Academy of Agriculture Sciences (CAAS), Beijing 100081, China; 2. CIMMYT China Office,;Investigation on Milling and Baking Quality of Chinese Spring-sown Wheat[J];Acta Tritical Crops;2002-01
3 YANG Fang-ping, WANG Sheng-rong ( Gansu Academy of Agricultural Sciences, Lanzhou 730070, China; Gansu Agricultural University, Lanzhou 730070, China);Relationship between the Subunits of High Molecular Weight Gluten and Quality Characters of Wheat[J];Acta Tritical Crops;2003-04
4 LIU Ping 1,2 , XU Jian Min 1, JIN Qian 2, WANG Yi Qing 2, ZHU Hong 2, ZHOU Yuan Cong 2 1Department of Hematology, Zhongshan Hospital, Fudan University, Shanghai, 200032 China; 2Institute of Biochemistry and Cell, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031 China;Purification and Characterization of Platelet Aggregation Inhibitor Component from Venom of Agkistrodon Halys Pallas[J];Journal of Experimental Hematology;2004-02
5 Li Wei Dang; et al(Dept of Immunology);Fast Purification of Human Recombinant Interleukin-8[J];Journal of Zhenjiang Medical College;1997-02
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LIU Yan-ling,TIAN Ji-chun (Agronomy college, Shandong Agricultural University,Taian 271018, China);Regression analysis of wheat quality traits to bread[J];Shandong Agricultural Sciences;2004-02
2 ZHANG Hua-wen,TIAN Ji-chun,LIU Yan-ling (Agronomy College, Shandong Agricultural University, Taian 271018, China);Characteristic and correlation analysis of grain quality among wheat varieties[J];Shandong Agricultural Sciences;2004-06
3 GUAN Yan-an~1,LI Yu-lian~2,WANG Xi-bo~2,SUI Xin-xia~1, HUANG Cheng-yan~(1*),LI Gen-ying~1,YANG Yan-bing~1 (1.Crop research institute, Shandong acdademy of agricultural sciences, Jinan,250100; 2.Laiyang Agricultural College, Laiyang, 261000);Diversity of Wheat High Molecular Weight Glutenin Subunits[J];Shandong Agricultural Sciences;2005-02
4 MU Qiu-huan,WU Ke,QIAN Zhao-guo,CONG Xin-jun(Taian Academy of Agricultural Sciences,Taian 271000,China);Study on Wheat Gluten Index Correlated with HMW-GS and Bread Toasting Quality[J];Shandong Agricultural Sciences;2009-07
5 CHEN Xiao-juan et al (College of Agronomy, Agricultural University of Hebei,Baoding, Hebei 071000);Research on the High Molecular Weight Glutenin Subunits And Industrial Quality of Wheat[J];Journal of Anhui Agricultural Sciences;2005-05
6 SUN Cui-hua et al(Huzhu Office of Weather Modification,Huzhu,Qinghai 100039);History and High-yielding Cultivation of Spring Wheat in Chaidamu Basin[J];Journal of Anhui Agricultural Sciences;2006-01
7 HU Yun et al (Institute of Wheat Culture,Guizhou University,Guiyang,Guizhou 550025);Study on the Subunit Composition of High Molecular Weight Glutenin in Wild Emmer[J];Journal of Anhui Agricultural Sciences;2008-01
8 LIU Yan hua,WANG Hong gang *,LIU Shu bing (Agronomy College of Shandong Agricultural University,Taian,Shandong 271018,China);The analysis of high molecular weight glutenin subunits compositions in wheat[J];Acta Botanica Boreali-occidentalia Sinica;2002-06
9 YANG Fangping,LI Wanghong(Gansu Academy of Agricultural Sciences,Lanzhou 730070,China);Genetic variation of HMW-GS among gansu wheat varieties[J];Acta Botanica Boreali-occidentalia Sinica;2003-05
10 YAN Hua-xiao~1,ZHAO Hui~(1*),WANG Xian-ze~2,WANG Fang~2(1 Department of Biotechnology,College of Chemical and Environmental Engineering,Shandong University of Science and Technology,Qingdao,Shandong 266510,China;2 Key Laboratory of Crop Biology of Shandong Province,Shandong Agricultural University,Tai'an,Shandong 271018,China);AS-PCR Marker of HMW-GS 1Bx14 Related to Baking Quality[J];Acta Botanica Boreali-Occidentalia Sinica;2006-08
【Co-references】
Chinese Journal Full-text Database 9 Hits
1 LU Xiao-mei, XU Xiang-yang, LI Jing-fuDepartment of Vegetable Breeding of Northeast Agricultural University, Harbin Heilongjiang 150030;Aduance of research on tomato fulvia fulva (cook) and its resistance breeding[J];Journal of Northeast Agricultural University;2002-04
2 CHEN Xusheng, 1CHEN Yongxuan, 2HUANG Junqi ( 1Department of Microbiology,Nanjing Agricultural University,Nanjing 210095; 2Institute of Industrial Crops,Jiangsu Academy of Agricultural Sciences,Nanjing 210014);Analysis of Amino Acid Component of Wilting PeaksProteins Produced by Three Verticillium dahliae Strains and Its Biochemical Properties[J];JIANGSU JOURNAL OF AGRICULTURAL SCIENCES;2000-01
3 LU Xin-yu1,LIU Ying-jie2,LIU Ying-li2,LI Li3,JIANG Min3(1.Institute of Fine Chemicals,Jiangsu Polytechnic University,Changzhou 213164,China;2.Changzhou Great New Material Technology CO.,LTD,Liyang 213372;3.The Second People′s Hospital of Wujin,Wujin 213011);Research on Separation and Purification of Potato Tyrosinase by FPLC Method[J];Journal of Jiangsu Polytechnic University;2007-04
4 DONG Zhuan-nian,GONG Xiu-hui,PENG Bo,LIU Xiao-zhu(Southwest Foresty College,Kunming 650224,China);Research of Purification the Peptides from Gastrodiae elata B1.[J];Biotechnology;2007-06
5 ZHAO Xiao-jun~1,WANG Mei-qin~2,LIU Hui-ping~2,HAN Ju-cai~2(1.Plant Protection Institute,Shanxi Agricultural Academy of Sciences,Taiyuan 030001,China;2.College of Agronomy,Shanxi Agricultural University,Taigu 030801,China);Influence of Different Strains′toxicant on Seeds Buding in Fulvia Fulva[J];Journal of Shanxi Agricultural Sciences;2005-01
6 LIU Hui-qin~1,LIU Hui-ping~2,HAN Ju-cai~2 (1. Department of Horticulture, Tianjin Agricultural College, Tianjin 300384, China; 2. Shanxi Agricultural University, Taigu 030801, Shanxi Province, China);Bioassay of Pathotoxin from Cladosporium fulvum[J];Journal of Tianjin Agricultural College;2004-01
7 LIU Hui qin 1,LIU Hui ping 2,HAN Ju cai 2,WANG Jian ming 2 (1 Department of Horticulture,Tianjin Agricultural College,Tianjin 300384,China; 2 College of Agronomy,Shanxi Agricultural University,Taigu,Shanxi 030801,China);Effect of toxin from Cladosporium fulvum on CAT and protective substance of tomato seedling[J];Journal of Northwest Sci-Tech University of Agriculture and Forestry;2005-05
8 LI Chun-xia,XU Xiang-yang,KANG Li-gong,LI Jing-fu(Horticultural College,Northeast Agricultural University,Harbin 150030,Heilongjiang,China);Monitoring the Variation of Physiological Races of Tomato Leaf Mold in Northeastern Three Provinces from 2006 to 2007[J];China Vegetables;2009-02
9 GAO Fen1,2,MA Li-ping1,QIAO Xiong-wu1,HAO Bian-qing1(1Shanxi Key Laboratory of Pesticide Science,Taiyuan 030031,China;2College of Plant Protection,Shenyang Agricultural University,Shenyang 110161,China);Purification of antifungal peptide produced by antagonistic Bacillus cereus BC98-I against Fusarium oxysporum[J];Acta Phytopathologica Sinica;2007-04
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Ma Chuanxi;Xu Feng and Chen Guowang(Department of Agronomy,Anhui Agricultural University);An Assessment of HMW Glutenin Subunits for the Prediction of Baking Quality in Bread Wheat Varieties[J];JOURNAL OF ANHUI AGRICULTURAL UNIVERSITY;1995-02
2 Hu Guangbiao Jia Zhian Zhou Qingzhen (Hongxinglong Institute of Heilongjiang Province);Research on Quality Changes of the Good quality Spring Wheat[J];HEILONGJIANG AGRICULTURAL SCIENCE;1998-03
3 ZHANG Yong 1, HE Zhong hu 1,2 , WANG Mei fang 3, ZHOU Gui ying 1, WANG De sen , ZHANG Yan 1 (1. Institute of Crop Breeding and Cultivation, Chinese Academy of Agriculture Sciences (CAAS), Beijing 100081, China; 2. CIMMYT China Office,;Investigation on Milling and Baking Quality of Chinese Spring-sown Wheat[J];Acta Tritical Crops;2002-01
4 ZHANG Xiao ke 1, LI Yao ke 2, WEI Yi min 1 (1. Northwest Sci Tech University of Agriculture and Forestry, Yangling, Shaanxi 712100, China; 2. Weicheng Crop Seed Farm, Weicheng District, Xianyang, Shaanxi 712034, China);Characterics and Genetic Transformation of Wheat Seed Storage Proteins[J];Acta Tritical Crops;2002-02
5 YAN Ben ju 1, REN Zheng long 2 (1.Department of Basic Science, Sichuan Agricultural University, Yaan 625014, Sichuan, China; 2.State Key Laboratory for Plant Genetics and Breeding, Sichuan Agricultural University, Yaan 625014, Sichuan, China);Structural Studies on the High-molecular-weight Subunits of Wheat Glutenin in Some Wheat Line[J];Journal of Sichuan Agricultural University;2001-04
6 ZHANG Xiang and ZHOU Yuan-Cong(Shanghai Institute of Biochemistry,Academia Sinica,200031)ZHUANG Qing-Qi(Department of Biochemistry, School of Pharmacy of Shanghai Medical University,200032);Mechanism of Inhibition of Platelet Aggregation by Acidic Phospholipase A2 from Agkistrodon halys Pallas[J];ACTA BIOCHIMICA ET BIOPHYSICA SINICA;1995-04
7 Zhang Huaigan,Chen Jixian,Zhao Xulan ( Northwest Plateau Institute of Biology , the chinese Academy of sciences,Xining 810001)Hu Han (Institute of Genetics , the Chinese Academy of Sciences. Beijing 100101);HMW-GS COMPOSITIONGSOF SPRNG WHEAT VARIETIES ON THE QINGHAI PLATEAU[J];ACTA AGRICULTURAE BOREALI-OCCIDENTALIS SINICA;1995-04
8 Zhao He Lu Shao-yuan Li Zong-zhi(Hebei Agricultural University,Baoding, 071001);Studies on Inheritance and Variation of HMW Glutenin Subunits and Their Correlation with Quality and Other Agronomic Characters in Wheat[J];Acta Agronomica Sinica;1994-01
9 Zeng Zhe-rong Li Ying-chan Sun Fang-hua Wang Guang-rui Zhou Gui-ying Pang Jia-zhi Lin Xiao-man (Institute of Crop Breeding and Cultivation. CAAS, Beijing 100081) Li Jian-hun Shi Xiu-yu Bao Si-jing Wu Zhi-xia (Cereal and Oil Crops Institute, Hebei Academy of ( Wheat Research Unit, Anyang, Institute of Crop Agricultural and Forestry Sciences. Breeding and Cultivation CAAS, Shijiazhuang 050031) Anyang. 455112);Evaluation of Bread Baking Quality and Cluster Analysis for 37 Wheat Varieties[J];Acta Agronomica Sinica;1994-06
10 LI Bao Yun WANG Yue Guang ** LIU Feng Ming *** SUN Hui LIU Guang Tian[WT6BX](Dept of Plant Genetics and Breeding, National Agricultural Univ of China, Beijing, 100094)[KH+2mmD][WT5HZ];Relationships between High Molecular Weight Glutenin Subunits (HMW GS) and Quality Traits in Wheat ( Triticum aestivum L.) *[J];Acta Agronomica Sinica;2000-03
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