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《Shandong Agricultural Sciences》 2009-06
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Verification of Authenticity for Pleurotus ostreatus Strains by Esterase Isozyme Analysis

ZHANG Qing-hua,ZHAO Xin-hai,ZHONG Li-juan,XU Chong(Liaoning Academy of Microbiological Sciences,Chaoyang 122000,China)  
The multiplicity of esterase isozyme of 19 Pleurotus ostreatus strains was studied using the vertical slab gel electrophoresis of polacrylamide in this paper.16 different bands of transport ratio and 17 types of enzyme bands were determined totally.The 19 strains were clustered into 6 large groups through the cluster analysis.The E2(0.172) band was the characteristic band of the first group.The E5(0.317) and E13(0.800) bands were the characteristic bands of the third group.Beiping4 and Shi2026,Beiping1 and Beiping9 were the same species respectively.The results showed that the multiplicity of Pleurotus ostreatus esterase isozyme was rich,and the differences between strains were significant,so it could be used for authenticity verification of Pleurotus ostreatus strains.
【Fund】: 辽宁省科技攻关项目(2006415004)
【CateGory Index】: S646.14
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