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《Shandong Agricultural Sciences》 2009-06
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Study on Polyacrylamide Gel Electrophoresis and Silver Staining Methods for Microsatellite DNA

ZHANG Xiu-hua,WANG Wen-feng(Department of Life Science and Technology,Xinxiang Medical University,Xinxiang 453003,China)  
In the study,the polymerase chain reaction(PCR)-polyacrylamide gel electophoresis and silver nitrate staining were used to detect DNA fragments.The results showed that using this method to detect DNA fragments was characterized as high sensitivity,slight stained background and clear bands.So using silver staining to analyze the polyacrylamide gel bands contributed to a longterm preservation and could reduce the pollution to human beings and environment.
【Key Words】: Polyacrylamide gel electrophoresis Silver staining Microsatellite DNA
【Fund】: 新乡医学院高学历人才科研启动项目(批准号:20040526)
【CateGory Index】: Q503
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【Citations】
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3 GUAN Hai-tao1,XU Shi-chang2,GUO Yu-hua1*(1.College of Agronomy,Shenyang Agricultural University,Shenyang 110161,China;2.Chinese Academy of Agricultural Sciences,Beijing 100094,China);Comparison of Two Ways of Silver Staining for DNA in PAGE[J];Journal of Shenyang Agricultural University;2006-01
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