Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Shandong Agricultural Sciences》 2009-07
Add to Favorite Get Latest Update

System Establishment of Tisue Culture in Vitro and Transgene Technology for Cucurbita ficifolia B.

LI Chang-sheng,ZHAO Chuan-zhi,LI Ai-qin,XIA Han(High-Tech Research Center,Shandong Academy of Agricultural Sciences/ Key Laboratory of Crop Genetic Improvement and Biotechnology,Huanghuaihai,Ministry of Agriculture/Key Laboratory for Genetic Improvement of Crop,Animal and Poultry of Shandong Province,Jinan 250100,China)  
With the cotyledon of Cucurbita ficifolia B.as explants,the tissue culture system was established.The highest frequency of shoot regeneration was observed from four-day-old cotyledon explants cultured on MS containing 1.0 mg/L BA.Elongated shoots were rooted on 1/2MS supplemented with 0.5 mg/L GA3.100 mg/L kanamycin or 50 mg/L hygromycin could effectively inhibit the regeneration of non-transgenetic shoots.
【Fund】: 山东省农业科学院青年基金项目(编号:2007YQN001)资助
【CateGory Index】: S642.1
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 5 Hits
1 ZHANG Wei-hua~1,SUN Xiao-lei~1,WANG Zhi-feng~1,CAO Qi-wei~1,LIU Wen-bao~2(1.Research Institute of Vegetable,Shandong Academy of Agricultural Sciences,Jinan 250100,China;2.Development Corporation of Agricultural Science and Technology,Jinan 250100,China);Study on the High Efficient Tissue Culture System of Pumpkin[J];Shandong Agricultural Sciences;2006-06
2 YU Yi-he et al(Horticulture Plant Tissue Culture Institute of College of Landscape,Henan Institute of Science and Technology,Xinxiang,Henan 453003);Study on Pumpkin Culture in Vitro and Regeneration[J];Journal of Anhui Agricultural Sciences;2006-20
3 ZOU Ke-qin et al (College of Life Science,China Jiliang University,Hangzhou,Zhejiang 310018);Effect of Different Plant Growth Regulators on Tissue Culture and Plantlet Regeneration of Pumpkin[J];Journal of Anhui Agricultural Sciences;2008-13
4 ZHANG Ya-Feng CAO Jia-Shu~* WU Tao Institute of Vegetable Science,Zhejiang University,Hangzhou 310029,China;Establishment of Regeneration System and Its Applications in Squashes(Cucurbita)[J];Plant Physiology Communications;2007-03
5 ZENG Yi-an, ZHU Yue-lin~(①), HUANG Bao-jian , Yang Li-fei (College of Horticulture,Nanjing Agricultural University, Nanjing 210095, China),;Effects of Cucurbita ficifolia as rootstock on growth, fruit setting, disease resistance and leaf nutrient element contents in Cucumis sativus[J];Journal of Plant Resources and Environment;2004-04
Chinese Journal Full-text Database 10 Hits
1 ZHANG Wei-hua1,ZHU Yan-yan2,WANG Zhi-feng1,CAO Qi-wei1,YU Yu-mei3,SUN Xiao-lei1*(1.Vegetable Institute of Shandong Academy of Agricultural Sciences, Jinan 250100,China;2.College of Horticulture Science and Engineering,Shandong Agricultural University,Taian 271018,China;3.Hunan Agricultural University,Changsha 410128,China);Studies on Optimizing Cucumber Regeneration System of Three Different Genotypes[J];Shandong Agricultural Sciences;2007-06
2 XU Heng-jian (College of Life Science,Shandong University of Technology,Zibo 255049);Effect of KT and NAA on the Regeneration of the Oucurbita ficifolia Bouch[J];Northern Horticulture;2007-04
3 CAI Zu-guo1,ZHOU Hui-ping2,ZHAO Yi-peng1,YANG Peng-ming1,ZHOU Jun-guo1(1.School of Landscape and Architecture,Henan Institute of Science and Technology,Xinxiang,Henan 453003,China;2.College of Economic and Management,Xinxiang Institute,Xinxiang,Henan 453003,China);Studies on the Rapid Propagation in Vitro of Disease Resistant Pumpkin Germplasm 482-2[J];Northern Horticulture;2009-01
4 ZOU Keqin ZHANG Yongjun LOU Jidong (College of Life Science,China Jiliang University,Hangzhou Zhejiang 310018);Progress of Cucurbita moschata Duch.[J];Journal of Changjiang Vegetables;2008-08
5 GAO Yankui1,CHEN Puhong2,LI Xin3,ZHAO Zhijun1,WANG Liping1 (1.College of Agriculture,Hebei University of Engineering,Handan 056021;2.Handan Middle Agricultural School;3. Cangzhou Normal School);Effects of Different Varities of Stocks on Yield and Fruit Quality of Cucumber’s Growth[J];Journal of Changjiang Vegetables;2008-18
6 ZHOU Jun-guo,LI Gui-rong,YANG Peng-ming(Horticultural Department of Henan College of Science and Technology,Xinxiang 453003,China);Quantitative character analysis and cluster analysis of 37 pumpkin (Cucurbita moschata Duch) inbred-lines[J];Journal of Agricultural University of Hebei;2006-04
7 CAI Zu-guo1,ZHOU Hui-ping2,ZHAO Yi-peng1,LI Xin-zheng1,YANG Peng-ming1(1.School of Horticulture and Landscape Architecture,Henan Institute of Science and Technology,Xinxiang 453003,China; 2.College of Economic and Management,Xinxiang Institute,Xinxiang 453003,China);Studies on the regeneration technique of disease resistant pumpkin germplasm 482-2 in vitro[J];Guangdong Agricultural Sciences;2007-12
8 LUO Xuan-zhao1,WEI Shi-hua2 (1.Baise Agriculture School,Baise,Guangxi 533000,China;2.Agricultural Service Center of Jiuzhou Twonship,Tianlin County,Guangxi 533000,China);New varieties introduction study of small type pumpkins for autumn growing in Tianlin County[J];Guangxi Agricultural Sciences;2007-04
9 Zhang Xuemei;Varieties Comparison Test of Green-skinned Pumkin[J];;2009-27
10 JIANG Yu1, 2, LI Haitao2, LIU Aiqun2 (1. College of Horticulture, Shenyang Agricultural University, Shenyang 110161, China; 2. Vegetable Research Institute, Liaoning Academy of Agricultural Sciences, Shenyang 110161, China);Study on the distant hybridization affinity and change of physiological and biochemical of Chinese and Indian pumpkins[J];Journal of Northeast Agricultural University;2009-08
China Proceedings of conference Full-text Database 4 Hits
1 ;嫁接对蔬菜抗逆性影响的研究进展[A];[C];2006
2 Liu Yi-sheng~1,Lin De-pei~2,Sun Xiao-wu~3,Wang Chang-lin~1 (1.Institute of Vegetables and Flowers,Chinese Academy of Agricultural Science,Beijing, 100081,China;2.Institute of Horticulture,Sichuan Academy of Agricultural Sciences, Chengdu,Sichuan,610066,China;3.Hunan Cucurbit Research Institute,Shaoyang, Hunan,422001,China);Advances on the cucurbit industry and cucurbit science and technology in China[A];[C];2009
3 LI Xin-zheng,DU Xiao-hua,ZHOU Jun-guo,LI Zhen-xia,SUN Yong-dong (Department of Horticulture,Henan Institute of Science and Technology,Xinxiang,China, 453003);The grey relational analysis of the main agronomic characters in Cucurbita moschata[A];[C];2009
4 FU Hong-Bing,CUI Chong-Shi ZHAO Xi,LIU Qi, (Horticulture College,Northeast Agricultural University,Harbin Helongjiang 150030);Establishment of Cucurbita Genetic Transformation System by Agrobacterium tumefaciens[A];[C];2009
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Wang Jie et al (Department of Plant Protection,Anhui Agricultural University,Hefei 230036);Studies on the Grafting Method of Seedless Watermelon and its Resistance to Virus[J];Journal of Anhui Agricultural Sciences;2002-05
2 GENG Xin-li et al (College of Horticulture,Xinjiang Agricultural University,Wulumuqi,Xinjiang 830052);Multiplication of Ornamental Pumpkin with the Method of Tissue Culture[J];Journal of Anhui Agricultural Sciences;2006-07
3 WANG Jian fei,WANG Hong mei,KAN Hai feng (1.Department of Teaching Affairs,Anhui Technical Teachers College,Fengyang 233100,China 2.Bengbu Luyu Agricultul Group,Anhui Bengbu,233000,China);Suudy on the Nutrient Characteristics of Nitrogen,Phosphorus and Potassium of Dutch-3 Cucumber with Substrate Culture[J];Journal of Anhui Technical Teachers College;2003-03
4 Jiang Youtiao; Zhang Mingfang; Sun Lixiang;Progress and Prospect in Cucurbits Culture by Grafting in China[J];JOURNAL OF CHANGJIANG VEGETABLES;1998-06
5 Chen Guilin;Nie Lanchun;Zhao Lin(Department of Horticulture, Hebei Agric Univ, Baoding 071001 );The Study on Growth and Mineral Concentrations in the Xylem Exudate of Grafted Watermelon[J];JOURNAL OF AGRICULTURAL UNIVERSITY OF HEBEI;1999-03
6 Shi Jiang and Le Jinhua (South China University for Tropical Agriculture ,Haikou, Hainan 571101 China; Bioengineering College , Shihezi University ,Shihezi, Xinjiang 832003 China);Research on Using the Segment of Cotolydon as Explant in vitro for Regeneration of Seed Watermelon[J];Journal of Fruit Science;2003-04
7 SHENG Yuping,WANG Aiqin,HE Longfei,TANG Xiangmin (Agric. College, Guangxi Univ., Nanning 530005, China);Propagation of Cucurbita moschata by tissue culture[J];Journal of Guangxi Agricultured and Biological Science;2002-03
8 Jiao Xiaoyan(Institute of Soil and Fertilizer, Shanxi Academy of Agricultural Sciences,Taiyuan)Chi Baoliang(Arid Farming Centre, Shanxi Acndemy of Agricultural Sciences,Talyuan)Cheng Jizhen(Institute of Vegatables, Shanxi Academy of Agricultural Sciences,;Studies on Characteristics of Nutritive Absorption and N, K Nutrition on Plant Growh of Cucumber in Sheltered Ground[J];JOURNAL OF SHANXI AGRICULTURAL SCIENCES;1997-03
9 NIE Lan chun, CHEN Gui lin ( Department of Horticulture, Hebei Agricultural University, Baoding 071001);Study on Growth Trends and Physiological Characteristics of Grafted Watermelon Seedlings[J];ACTA AGRICULTURAE BOREALI-OCCIDENTALIS SINICA;2000-01
10 ZOU Jian1, SONG Ming1, TANG Qing-lin1, ZHANG Zhong-ling2, LIU Hong-yu2, ZHOU Hong2(1. Department of Horticulture, Southwest Agricultural University, Chongqing 400716, China; 2. Chongqing Bureau of Agriculture, Chongqing 400010, China);PRELIMINARY STUDY OF IN VITRO CULTURE OF COTYLEDON EXPLANTS OF ORNAMENTAL CUSHAW (CUCURBITA MOSCHATA)[J];Journal of Southwest Agricultural University;2003-04
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved