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《Shandong Agricultural Sciences》 2009-07
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System Establishment of Tisue Culture in Vitro and Transgene Technology for Cucurbita ficifolia B.

LI Chang-sheng,ZHAO Chuan-zhi,LI Ai-qin,XIA Han(High-Tech Research Center,Shandong Academy of Agricultural Sciences/ Key Laboratory of Crop Genetic Improvement and Biotechnology,Huanghuaihai,Ministry of Agriculture/Key Laboratory for Genetic Improvement of Crop,Animal and Poultry of Shandong Province,Jinan 250100,China)  
With the cotyledon of Cucurbita ficifolia B.as explants,the tissue culture system was established.The highest frequency of shoot regeneration was observed from four-day-old cotyledon explants cultured on MS containing 1.0 mg/L BA.Elongated shoots were rooted on 1/2MS supplemented with 0.5 mg/L GA3.100 mg/L kanamycin or 50 mg/L hygromycin could effectively inhibit the regeneration of non-transgenetic shoots.
【Fund】: 山东省农业科学院青年基金项目(编号:2007YQN001)资助
【CateGory Index】: S642.1
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