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《Shandong Agricultural Sciences》 2011-06
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Construction of Plant Expression Vector of Amaranthus caudatus Agglutinin Gene(ACA)

TIAN Hua-ying1,PANG Cai-hong2,XIA Yang2*,BAO Ying1,XIAN Yang2, LI Shuang-yun2,LI Li2,LIU Cui-lan2,MAO Xiu-hong2,YAN Li-ping2(1.College of Life Science,Qufu Normal University,Qufu 273165,China;2.Shandong Provincial Key Laboratory of Forest Tree Genetic Improvement/ Shandong Academy of Forestry,Jinan 250014,China)  
Amaranthus caudatus agglutinin(ACA),a kind of storage protein existed in the seeds of Amaranthus caudatus,belonged to amaranth lectin family and had lethal effect to homopteran pests such as aphid,Nilaparvata lugens and leafhopper.The vector pCAMBIA2300-35S-OCS contained the 35S promoter and its termination sequence,kanamycin(Kan) resistant gene NPT Ⅱ,and the vector pGEMT-ACA contained the ACA gene.The ACA gene was amplified by polymerase chain reaction(PCR) from the vector.After cleavage by KpnⅠand SalⅠand linkage by ligase,the plant expression vector pCAMBIA2300-ACA was constructed.Then the vector was transformed into the Agrobactrium tumefaciens GV3101 and LBA4404 for preservation.
【Fund】: 济南市高校院所自主创新计划“优良城市园林绿化树种香樟转基因抗寒新种质选育”;; 山东省农业良种工程“国槐、香樟耐盐抗旱抗寒转基因新品种选育”课题资助
【CateGory Index】: S336
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【Citations】
Chinese Journal Full-text Database 5 Hits
1 LIU Zhao_Hua 1,2 , GUO Hong_Nian 1 , ZHENG Guang_Yu 2 and TIAN Ying_Chuan 1* 1 Institution of Microbiology Chinese Academy of Sciences, National Key Laboratory of Plant Genomics, Beijing 100080, China 2 Beijing Normal University, Beijing 100875, China;Cloning of ACA Gene Promoter and Preliminary Study of its Function[J];Chinese Journal of Biotechnology;2005-01
2 GAO Ying QU Li-jia CHEN Zhang-liang National Laboratory of Protein Engineering and Pant Gene Engineering, 100871;Study on the Molecular Biology of Plant Lectin[J];BIOTECHNOLOGY INFORMATION;2000-05
3 Wang Zhiben Li Xueyong Guo Sandui(Biotechnology Research Center Chinese Academy of Agriculture Sciences 100081);Plant Lectins and Anti-insect Genetic Engineering[J];BIOTECHNOLOGY INFORMATION;1998-02
4 Pang Caihong et al.;Construction of plant expression vector of phosphatidyliositol synthase gene PIS[J];Journal of Shandong Forestry Science and Technology;2010-04
5 LIU Zhao-Hua~1,2,ZHANG Zhen-Shan~1,GUO Hong-Nian~1,JIA Yan-Tao~1, ZHENG Guang-Yu~2,TIAN Ying-Chuan~1,① (1.Institution of Microbiology,Chinese Academy of Sciences, National Key Laboratory of Plant Genomics,Beijing 100080,China; 2.College of Life Sciences,Beijing Normal University 100875,China);Expression of Two Plant Agglutinin Genes in Transgenic Tobacco Plants[J];Acta Genetica Sinica;2005-07
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Li Changfu et al (Dept. of Plant Protection, Anhui Agricultural University, Hefei 230036 );Progress on Testing and Identification of Resistance to Cotton Boll Worm of Bt Transgenic Cotton[J];Journal of Anhui Agricultural Sciences;2002-03
2 LI Feng-ling et al (Rice Research Institute of Sichuan Agricultural University,Wenjiang,Sichuan 611130);Plant Lectin and its Application in the Genetic Engineering of Plant Resistance to Insects[J];Journal of Anhui Agricultural Sciences;2006-03
3 ZHU Yue (Biology Department of Chifeng College,Chifeng 024000,China);The Function and Application of Agglutinin[J];Fisheries Science;2005-12
4 GAO Baojia,LI Chuan,LIU Junxia (Agricultural University of Hebei, Baoding 071001,China);The effect of transgenic insect-resistant forst plant on the insect pest control and its approach of reasonable utilization[J];Journal of Agricultural University of Hebei;2003-04
5 LUO Ying ying, LIANG Yue rong [WT6BX](Department of Tea Science, Zhejiang Uinversity, Hangzhou 310029, China )[WT5”HZ];Studies on the Construction of Bt Gene Expression Vector and Its Transformation in Tea Plant[J];JOURNAL OF TEA SCIENCE;2000-02
6 Zhang Shuang(Northeast Forestry University, Harbin 150040, P. R. China); Du Kejiu(Hebei Agricultural University); Li Chengde(Northeast Forestry University); Su Jinhao(Baoding Vocational and Technical College);Research Development of Gene Engineering on Resistance to Coleopterans[J];Journal of Northeast Forestry University;2004-05
7 DU Li1,ZENG Xiao-hui1,ZHOU Suo1,BAO Man-zhu2(1 Shool of Life Science and Technology,Nanyang Normal University,Nanyang,He'nan 473000,China;2 Key Laboratory of Horticultural Plant Biology of Ministry of Education,College of Horticulture and Forestry Sciences,Huazhong Agricultural University,Wuhan 430070,China);Transfer of Green Fluorescent Protein Gene into Embryogenic Calluses of Cinnamomum camphora[J];Acta Botanica Boreali-Occidentalia Sinica;2008-03
8 ZHAO Qing,LI Dong (Key Laboratory of Animal Virology of ministry of Agriculture,National Foot-and-mouth Disease Reference Laboratory of China,State Key Laboratory of Veterinary Etiologic Biology,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou,Gansu,730046,China);Viral Receptors and Its Research Status[J];Progress in Veterinary Medicine;2009-07
9 Zheng Jin Liu Kaiyu Hong Huazhu (Central China Normal University, College of Life Wuhan 430079);Progress in Research on Poplars Resistance by Transgenic Method[J];Hubei Forestry Science and Technology;2004-01
10 QI Jian-min1,2,XU Jian-tang1,LIN Li-hui2,TAO Ai-fen2,LAN Tao2,WU Jian-mei2(1.College of Life Science;2.College of Crop Science,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China);Molecular verification of insect-resistant transgene progeny of kenaf variety Fuhong 952[J];Journal of Fujian Agriculture and Forestry University(Natural Science Edition);2008-01
China Proceedings of conference Full-text Database 4 Hits
1 ;Experimental Comparisons of Bioassays of Myzus Persicae Methods for Detached Tobacco Leaf[A];[C];2008
2 Jiao Xiaoguo Zhang Guoan Tu Jumin Cui Xuhong (Central China Agriculture University,Wuhan,China 430070);Bioassay on resistance to pests of the transgenic Bt rice[A];[C];2002
3 Wang Cai-lin Zhao Ling Zhu Zhen Zhang Ya-dong Lin Jing Zhang Suo-bing Chen Tao Liu Xian-jin Wang Dong-Ian Huang Jun-qi (Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China) Gong Zhen-zhen (Shanghai Institute of Biochemistry and cell Biology, Academy Sinica, Shanghai, 200031, China);Transgenic Rice Plant Transformed by Introduction of Arrowhead Proteinase Inhibitor (API) Geneinto Rice by Pollen-tube Pathway Method[A];[C];2004
4 CHAI Hong-mei YAO Chun-xing ZHANG Shao-song HUANG Xing-qi~*(Biotechnology Institute,Yunnan Academy of Agricultural Sciences,Kunming 650223,China);Construction of plant expression vector containing GNA gene and its transformation into tobacco[A];[C];2004
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Tian Chao;Wang Jian and Wu Zhengting(Anhui Agricultural University,Hefei 230036);The Recent Advances of Molecular Biology Applied in Insect Pest Control[J];JOURNAL OF ANHUI AGRICULTURAL UNIVERSITY;1995-04
2 ShiBaohua;FuRunmin;ZhaoZhengyang and Shui Shouqi(Shaanxi Academy of Agricultural Sciences,Yangling, Shaanxi 712100);STUDY OF REGENERATION CAPABILITY FROM APPLE CULTIVAR LEAF TISSUE[J];ACTA BOTANICA BOREALI-OCCIDENTALIA SINICA;1995-01
3 Zheng Jin Liu Kaiyu Hong Huazhu (Central China Normal University, College of Life Wuhan 430079);Progress in Research on Poplars Resistance by Transgenic Method[J];Hubei Forestry Science and Technology;2004-01
4 WU Lijun(Fujian Academy of Forestry,Fuzhou 350012,China);Application and Limitations of the Woody Plant Tissue Culture Technique in the Forestry Scientific Research and Production[J];Journal of Fujian Forestry Science and Technology;2003-01
5 Zeng Lihui and LüLiuxin (Department of Horticulture,Fujian Agriculture and Forestry University,Fuzhou,Fujian350002);Advances in Research on Genetic Transformation in Woody Fruit Crops[J];Journal of Fruit Science;2002-03
6 Peng Minzhang and Deng Jiusheng; Gan Lin(Guangxi State Farming University,Nanning,530227)(Hunan Agricultural University);Preservation of Avocado in vitro Under Low Temperature[J];JOURNAL OF FRUIT SCIENCE;1996-02
7 He Hong Pan Ruichi (Department of Biology, South China Normal University, Guangzhou 510631) Han Meili Li Gengguang (South China Institute of Botany, Academia Sinica, Guangzhou 510631);The study on the factors affecting embryogenesis of Citrus sinensis Osbeck cv. Hongjiang[J];GUIHAIA;1998-04
8 ;PROCEEDING IN ANTI-INSECT GENETIC ENGINEERING OF POPLAR[J];Hebei Journal of Forestry and Orchard Research;2001-01
9 Chen Zhankuan Zhi Yubao Yi Minglin Wang Jinlan Liang Xiuyin Tu Lichuan (Henan Academy of Agrcultural Sciences,Zhengzhou 450002) Fu Rongzhao Cao Guangcheng Shi Yanhong Sun Yongru [WT5”BZ〗(Institute of Genetics,Academi;Transformation of Engineered Male Sterile Gene and Establishment of Transgenic Plants in Sesame (Sesamum indicum L.)[J];ACTA AGRICULTURAE BOREALL—SINICA;1996-04
10 Jiang Xi-wang 1,2 ,Bao Man-zhu 1 (1 College of Horticulture and Forestry Sciences,Huazhong Agricultural University,Wuhan 430070,China; 2 College of Medicine and Life Sciences,Jianghan University,Wuhan 430056,China);Rapid PCR identifying of transgenic plants of chrysanthemum integration Bt gene and GNA gene[J];Hubei Agricultural Sciences;2003-02
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