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《Shandong Agricultural Sciences》 2011-06
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Construction of Plant Expression Vector of Amaranthus caudatus Agglutinin Gene(ACA)

TIAN Hua-ying1,PANG Cai-hong2,XIA Yang2*,BAO Ying1,XIAN Yang2, LI Shuang-yun2,LI Li2,LIU Cui-lan2,MAO Xiu-hong2,YAN Li-ping2(1.College of Life Science,Qufu Normal University,Qufu 273165,China;2.Shandong Provincial Key Laboratory of Forest Tree Genetic Improvement/ Shandong Academy of Forestry,Jinan 250014,China)  
Amaranthus caudatus agglutinin(ACA),a kind of storage protein existed in the seeds of Amaranthus caudatus,belonged to amaranth lectin family and had lethal effect to homopteran pests such as aphid,Nilaparvata lugens and leafhopper.The vector pCAMBIA2300-35S-OCS contained the 35S promoter and its termination sequence,kanamycin(Kan) resistant gene NPT Ⅱ,and the vector pGEMT-ACA contained the ACA gene.The ACA gene was amplified by polymerase chain reaction(PCR) from the vector.After cleavage by KpnⅠand SalⅠand linkage by ligase,the plant expression vector pCAMBIA2300-ACA was constructed.Then the vector was transformed into the Agrobactrium tumefaciens GV3101 and LBA4404 for preservation.
【Fund】: 济南市高校院所自主创新计划“优良城市园林绿化树种香樟转基因抗寒新种质选育”;; 山东省农业良种工程“国槐、香樟耐盐抗旱抗寒转基因新品种选育”课题资助
【CateGory Index】: S336
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