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《Shandong Agricultural Sciences》 2011-12
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SSR Linkage Map Construction and QTL Identification for Resistance Gene of MRDV in Maize

SHANG Wei1,ZHANG Yan-jun1,WEI Hai-zhong1,KONG Xiao-min2,JIANG Fei2,LIU Bao-shen1(1.State Key Laboratory of Crop Biology/ Agronomy College of Shandong Agricultural University,Taian 271018,China; 2.Jining Institute of Agricultural Sciences,Jining 272031,China)  
A mapping population consisting of 205 F5∶6 recombinant inbred lines(RILs) derived from the cross between maize inbred lines 80007 and 80044 was used in this study.A genetic linkage map was constructed containing 173 SSR markers,which covered 919.87 cM of the maize genome with an average distance of 5.32 cM between markers.The results showed that five QTLs were identified for MRDV resistance using inclusive composite interval mapping method(ICIM),which were located on chromosome 1,2 and 5 in Taian and explained a range of phenotypic variation from 4.69% to 17.74%;three QTLs for MRDV resistance were mapped on chromosome 1 and 2 in Feicheng,explaining phenotypic variation ranging from 4.95% to 9.13%.One common QTL detected on chromosome 2 was identified in both environments,explaining 17.74% and 8.76% of the phenotypic variation respectively,which could be used for fining mapping and positional cloning.
【Fund】: 转基因生物新品种培育重大专项(2009ZX08003-013B);; 山东省良种产业化项目[鲁科农字(2010)117号];; 农业生物资源创新利用研究子任务“专用玉米种质创新与利用”;; 山东省科技发展计划项目(2009GG10009021)资助
【CateGory Index】: S513
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