Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Shandong Agricultural Sciences》 2013-01
Add to Favorite Get Latest Update

Construction of Monocotyledon Expression Vector pUN3300

Li Zhen,Wang QingGuo,Sun Ping,Liu Wei(High-Tech Research Center,Shandong Academy of Agricultural Sciences/ Shandong Provincial Key Laboratory of Crop Genetic Improvement,Ecology and Physiology,Jinan 250100,China)  
Plant binary expression vector has an important role in plant genetic engineering research.The structural elements which are included in the expression vector,such as promoter,multiple cloning sites,selection marker,have important influences on the efficiency of plant genetic transformation,intensity and stability of exogenous gene expression.In order to improve the efficiency of exogenous gene to target monocotyledon by genetic transformation,the commercialized expression vector pCAMBIA3300 was used and modified in this study.Using incompletely digestion technology,the ubiquitin promoter was successfully harvested and subcloned into pCAMBIA3300.The new constructed plant binary expression vector was named as pUN3300.The new vector of pUN3300 will be in favor of monocotyledon transformation and laid basis for the function analysis of exogenous genes,improvement of plant properties,and new variety breeding.
【CateGory Index】: Q943.2
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 7 Hits
1 LI Hai-qing1,2,3,ZOU Min4,YAO Fang-yin1,2,LIU Wei1,2 (1.High-Tech Research Center,Shandong Academy of Agricultural Sciences/Key Laboratory of Crop Genetic Improvement and Biotechnology,Shandong Province,Jinan 250100,China;2.Key Laboratory of Crop Genetic Improvement and Biotechnology,Huanghuaihai,Ministry of Agriculture,Jinan 250100,China;3.College of Life Science,Shandong Normal University,Jinan 250014,China;4.The First Middle School in Laiwu,Laiwu 271100,China);Advances in Agrobacterium-Mediated Co-Transformation Systems for Marker-Free Transgenic Rice[J];Shandong Agricultural Sciences;2011-07
2 GUO Zhi-hong1,WANG Hanning1,TAO Lin2,ZHANG Jin-wen1,BAI Bin1,CHEN Zheng-hua3 (1. Agronomy Faculty of Gansu Agricultural University, Gansu,Lanzhou 730070; 2. College of Enviromental and Municipal Engineering, Gansu, Lanzhou 730070;3. Research Station for Postdoctors of Yasheng Group, Beijing 100101, China);Construction of ubi promoter-based plant expression vector of stilbene synthase gene and transformation of type Ⅱ callus of maize by particle bombardment[J];Journal of Gansu Agricultural University;2004-02
3 LI Hai-qing1,2,3,LIU Xu2,3,WANG Qing-guo2,3,YAO Fang-yin2,3,LIU Wei1,2,3(1.High-Tech Research Center,Shandong Academy of Agricultural Sciences,Key Laboratory of Crop GeneticImprovement and Biotechnology,Shandong Province,Jinan 250100,China;2.Key Laboratory of Crop GeneticImprovement and Biotechnology,Huanghuaihai,Ministry of Agriculture,Jinan 250100,China;3.College of Life Sciences,Shandong Normal University,Jinan 250014,China);Construction of Biosafe Vectors of Resveratrol Synthase Gene and Rice Genetic Transformation[J];Acta Agriculturae Boreali-Sinica;2011-02
4 LIU Hong-yan, MI Xiao-ju, CUI Ji-zhe (Department of Biology, Harbin Normal University, Harbin 150025, China);Characteristics and safety of bar gene, PAT proteins and glufosinate[J];Chinese Journal of Ecology;2007-06
5 LIU Ya,LI Jing-na,REN Wen,LI Xiang Maize Research Center,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China;Progress on Expression Vector for Plant Genetic Transformation[J];Current Biotechnology;2011-01
6 ZHANG Chang-Wei1,2, LING Ying-Hua1, SANG Xian-Chun1, LI Ping1, ZHAO Fang-Ming1, YANG Zheng-Lin1, LI Yun-Feng1, FANG Li-Kui1, and HE Guang-Hua1, 1 Chongqing Key Laboratroy of Application and Safety Control of Genetically Modified Crops, Southwest University, Chongqing 400716, China; 2 Rice and Sorghum Research Institute, Sichuan Academy of Agricultural Sciences, Luzhou 646000, China;Transgenic Rice Lines Harboring McCHIT1 Gene from Balsam Pear (Mo-mordica charantia L.) and Their Blast Resistance[J];Acta Agronomica Sinica;2011-11
7 HAN Kai1,2,WENG Jian-feng2,XUE Ji-quan1,XIE Chuan-xiao2,et al.(1.College of Agriculture,Northwest A&F University,Yangling 712100; 2.Institute of Crop Science,Chinese Academy of Agricultural Sciences / Crop Gene Resources and Genetic Improvement,National Key Science and Engineering,Beijing 100081,China);A Rapid and High Efficient Method to Construct Plant Expression Vectors[J];Journal of Maize Sciences;2012-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 WANG Xiang-chun et al(Yunnan Agricultural University,Kunming,Yunnan 650201);Advances in Selection Marker Genes of Plant Transformation[J];Journal of Anhui Agricultural Sciences;2011-22
2 QIAN Shan et al(Bioengineering College,Xihua University,Chengdu,Sichuan 610039);Synthesis of 3-Ethoxy-4-Ethoxy-Carbonyl-Phenylacetic Acid[J];Journal of Anhui Agricultural Sciences;2011-29
3 REN Yan-rong et al(Department of Biological and Chemical Engineering,Chongqing Education College,Chongqing 400067);Detection Method and Pharmacological Functions of Resveratrol[J];Journal of Anhui Agricultural Sciences;2012-02
4 WEI Jun-jie et al(Baoding University,Baoding,Hebei 071001);Study of Herbicide-resistant GM Maize[J];Journal of Anhui Agricultural Sciences;2012-14
5 ZHAO Yan-peng1,LI Jie1,LIU Qi2,XIA Shan-yong2,WU Li-cheng2,WANG Xiu-jun2,LI Xi-chen2(1.Life Science College of Northeast Agricultural University,Harbin 150030;2.Biotechnology Research Institute of Heilongjiang Academy of Agricultural Sciences,Harbin 150086,Heilongjiang,China);Construction of a Plant Expression Vector Containing Two T-DNA Based on phy Gene[J];Soybean Science;2010-03
6 LI Yong-guang,HUANG Wen-jia,ZHOU Shi,WANG Peng-peng,LI Wei-na,WANG Tao,LI Wen-bin(Key Laboratory of Soybean Biology in Chinese Ministry of Education,Agronomy college,Northeast Agricultural University,Harbin 150030,Heilongjiang,China);Sensitivity of Soybean to Herbicide Glufosinate[J];Soybean Science;2011-05
7 FENG Yu-lan~(1,2),WANG Han-ning~(1),ZHANG Jin-wen~(1,2),KONG Wei-ping~(3)(1.College of Agronomy,Gansu Agricultural University,Lanzhou 730070;2.Analysis Center,Gansu Agricultural University,Lanzhou 730070;3.Funong High-tech Seeds Limited Company of Gansu,Lanzhou 730070,China);Construction of monocotyledonous high-effective plant expression vector and establishment of regeneration system of maize immature embryos[J];Journal of Gansu Agricultural University;2006-06
8 ZHANG Zhen1,LI Sheng2,LIU Yuan1,LI Ting1,MA Shao-ying3,ZHANG Qing-song1,YANG Guang-xing1,WU Yuan-yuan1,FANG Yan1,LUO Li-yuan1(1.College of Biological Science and Technology,Gansu Agricultural University,Lanzhou 730070,China;2.Institute of Cytobiology,Lanzhou University,Lanzhou 730000,China;3.Lanzhou Huitong Biotechnology Co.,Ltd.Lanzhou 730000,China);Application of biotechnology in research of resveratrol[J];Journal of Gansu Agricultural University;2008-01
9 Li Jianrong 1,2 Zeng Xiaofang 2,3 Ai Shanshan 1,2 Zhao Degang 1,2,3* 1 College of Life Sciences, Guizhou University, Guiyang, 550025; 2 Guizhou Key Laboratory of Agricultural Bioengineering, Guiyang, 550025; 3 Key Laboratory of Green Pesticide and Agricultural Biological Engineering, Ministry of Education, Guiyang, 550025;Genetic Transformation of Rice Landrace“Guizhou HE Lailong”with CHI- PAT Bivalent Genes[J];Genomics and Applied Biology;2012-03
10 HUANG Qian 1a,2 ,LING Yan 1a ,JIANG Xian-bin 1b ,LONG Li-ping 1b* ,HUANG Feng-kuan 1a , HUANG Suo-sheng 1a ,WU Bi-qiu 1a (1a Plant Protection Research Institute; 1b Rice Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007,China; 2 Agricultural College,Guangxi University,Nanning 530005,China);Impacts of transgenic rice with Bar gene and glufosinate on feeding and oviposition behavior of brown planthopper, Nilaparvata lugens (Stl)[J];Journal of Southern Agriculture;2013-07
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 ZENG Jian-jun et al (College of Life Science,Jinggangshan College,Jian,Jiangxi 343009);Review and Prospect of Safety Research of Transgenic Crops[J];Journal of Anhui Agricultural Sciences;2007-22
2 WANG Ru-ping et al (Yangzhou Vocational College of Environment and Resources,Yangzhou,Jiangsu 225127);Biosafety Evaluation of Transgenic Products[J];Journal of Anhui Agricultural Sciences;2008-36
3 DENG Qi-ming et al(Rice Research Institution,Sichuan Agricultural University,Chengdu,Sichuan 611130);Study Progress on Rice Blast Resistance Gene Researches and Its Application in Blast Resistance Breeding[J];Journal of Anhui Agricultural Sciences;2009-04
4 Huang Yihong1* Liu Chunguang2 Ma Hongxiang1 Liu Genqi2 Zhou Miaoping1 Sun Yongru2 Yu Guihong1 Lu Weizhong11 Institute of Agrobiological Genetics and Physiology, Jiangsu Academy of Agricultural Sciences, Nanjing, 2100142 Institute of Genetics and Developnental Biology, Chinese Academy of Sciences, Beijing, 100101;Wheat Transformation by Pollen-Tube Pathway and High Effective Screening System[J];Molecular Plant Breeding;2004-06
5 Tan Yanning Yi Zili* Jiang Jianxiong Qin Jingping Xiao Liang The Key Lab of Cell Engineering, Hunan Agricultural University, Changsha, 410128;Strateges and Advances in Improving Resistance to Rice Blast by Transgenic Approaches[J];Molecular Plant Breeding;2004-06
6 Lin Chunjing1,2 Dong Yingshan1* Lin Xiufeng1 Wang Jichun3 Tian Wenzhong4 Chu Chengcai41 Center of Agri-Biotechnology, Jilin Academy of Agricultural Sciences, Gongzhuling, 136100; 2 Institute of Genetics and Cytology, Northeast Normal University, Changchun, 130024; 3 Institute of Plant Protect, Jilin Academy of Agricultural Sciences, Gongzhuling, 136100; 4 Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101;Primary Studies on Inheritance and Expression of Resistance to Rice Blast in Transgenic Rice[J];Molecular Plant Breeding;2006-02
7 Xu Huaxue 1 Xiong Jianhua 1, 2 Fu Binying 1* 1 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081; 2 College of Life Sciences, Wuhan University, Wuhan, 430072;Construction of RNAi Vectors for OsDAD1 Gene of Rice Using Gateway Technology[J];Molecular Plant Breeding;2007-01
8 Kong Zheng 1 Zhao Degang 1,21 Guizhou Key Laboratory of Agricultural Bioengineering, College of Life Sciences, Guizhou University, Guiyang, 550025; 2 Key Laboratory of Green Pestiside and Bioengineering of Education Ministry, Guiyang, 550025;The Combination of CHI and AFP Genes Introduced into Ryegrass Mediated by Agrobacterium[J];Molecular Plant Breeding;2008-02
9 Guo Jingnan 1 ,Liu Chonghuai 1 ,Pan Xing 1 ,and Wang Jishuan 2 ( 1 Zhengzhou Fruit Research Institute,CAAS,Zhengzhou,Henan450009; 2 Yanshi Agricultural Science and Technology Extension Centre,Yanshi,Henan471900);Advances in Research on Resveratrol in Vitis spp.[J];Journal of Fruit Science;2002-03
10 GUO Zhi-hong1,WANG Hanning1,TAO Lin2,ZHANG Jin-wen1,BAI Bin1,CHEN Zheng-hua3 (1. Agronomy Faculty of Gansu Agricultural University, Gansu,Lanzhou 730070; 2. College of Enviromental and Municipal Engineering, Gansu, Lanzhou 730070;3. Research Station for Postdoctors of Yasheng Group, Beijing 100101, China);Construction of ubi promoter-based plant expression vector of stilbene synthase gene and transformation of type Ⅱ callus of maize by particle bombardment[J];Journal of Gansu Agricultural University;2004-02
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved